User:Karlena L. Brown/Notebook/PVOH Research/2012/10/31: Difference between revisions
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==Ion Diffusion & Ion Selective Electrode Film Procedures== | ==Ion Diffusion & Ion Selective Electrode Film Procedures== | ||
# Cut | # Cut small piece of PVOH 22K film containing 1mL glutaldehyde exchanged in CuSO<sub>4</sub> for 10 min | ||
# Place small cut piece of PVOH 22K film in plastic double graduated cylinder apparatus | |||
'''Ensure the hole separating the two graduated cylinder columns is completely shielded and covered by the PVOH 22K film''' | |||
# Close and seal plastic double graduated cylinder apparatus | |||
# Next, in one graduated cylinder column pour in ~ 20mL of HPLC Grade H<sub>2</sub>O | |||
# Then, in other graduated cylinder column pour in ~20mL of the varied ion concentrated solution | |||
# Plug the electrodes into the SympHony meter and turn on the ion selective electrode instruments | |||
# Then press the arrows until ISE (ion selective electrode) and μS/cm (microsemen per centimeter) appear on the screen | |||
# Remove the electrodes from the storage solutions | |||
# Rinse the electrodes with distilled H<sub>2</sub>O and thoroughly dry both with KimWipes | |||
# Place each electrode directly into the graduated cylinder columns containing either the HPLC Grade H<sub>2</sub>O or the varied ion solution for analysis (one electrode per column) | |||
# Measure the total amount of solution available and present for each graduated cylinder column | |||
# Let each solution and electrode equilibrate for a set period of time (between 2-5 minutes) and then press measure on each instrument | |||
# Once the measurement is finalized, record the values observed for each solution | |||
==ISE Film Analysis: Varied Ion Solution== | ==ISE Film Analysis: Varied Ion Solution== |
Revision as of 02:40, 24 November 2012
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OBJECTIVES
Furfural Film Preparation
(PVOH 22K Actual Mass = 1.0029g)
(Furfural Actual Mass = 0.3192g)
Films Examined using FTIRRECALL FTIR PROCEDURAL PROTOCOL 9/17/12 Ion Diffusion & Ion Selective Electrode Film Procedures
Ensure the hole separating the two graduated cylinder columns is completely shielded and covered by the PVOH 22K film
ISE Film Analysis: Varied Ion SolutionA crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO4 solution was used for an Ion Selective Electrode Analysis.
ISE Film Analysis: HPLC Grade H2O SolutionA crosslinked PVOH 22K film containing 1mL glutaldehyde that had been exchanged for 10 min in the CuSO4 solution was used for an Ion Selective Electrode Analysis.
Notes
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