User:Karlena L. Brown/Notebook/PVOH Research/2012/10/26

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OBJECTIVES

• Make porphyrin solutions with varied SO42- salts
• Take UV-Vis of varied porphyrin solutions with diluted varied SO42- salts

Porphyrin Salt Selection and Preparations 2

SO42- salts used:Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4

Preparing 10mM Stock Solutions For Each Salt :

1. Obtain four clean 100mL volumetric flasks with stoppers
2. Measure out calculated amounts of each salt to establish 10mM concentration
5. Swirl each flask thoroughly for ~ 5 minutes to begin the dissolving process

(Ensure that the volumetric calibration line is not surpassed or solution must be remade.)

10mM Salt Solution Calculations

• 10mM Fe2(SO4)3 Solution
```  MW Fe2(SO4)3: 278.91 g/mol
10mM Fe2(SO4)3 × (100mL H2O / 1000mL H2O) × (278.91g Fe2(SO4)3 / 1 mol Fe2(SO4)3) = 0.2789g Fe2(SO4)3

```
• 10mM Al3(SO4)3 Solution
```  MW Al3(SO4)3: 356.37 g/mol
10mM Al3(SO4)3 × (100mL H2O / 1000mL H2O) × (656.37g Al3(SO4)3 / 1 mol Al3(SO4)3) = 0.6564g Al3(SO4)3

```
• 10mM FeSO4 Solution
```  MW FeSO4: 278.01 g/mol
10mM FeSO4 × (50mL H2O / 1000mL H2O) × (278.01g FeSO4 / 1 mol FeSO4) = 0.1390g FeSO4

```
• 10mM Na2SO4 Solution
```  MW Na2SO4: 142.04 g/mol
10mM Na2SO4 × (100mL H2O / 1000mL H2O) × (142.04g Na2SO4 / 1 mol Na2SO4) = 0.1420g Na2SO4

```
 SO42- Salts Selected Salt Mass Needed (g) Actual Salt Mass (g) HPLC Grade H2O Added (mL) Total Concentration(mM) Fe2(SO4)3 0.2879 0.3100 100 10 Al3(SO4)3 0.6564 0.6556 100 10 FeSO4 0.1390 0.1394 100 10 Na2SO4 0.1420 0.1464 100 10

Notes

• The Al3(SO4)3 10mM solution was very opaque and cloudy with some precipitate slightly falling out of solution. Before using the Al3(SO4)3 solution, it had to be swirled to ensure that all precipitate was in solution.
• Also for the FeSO4 10mM salt solution, it was prepared in a 50mL volumetric flask instead of a 100mL volumetric flask.
• Also for the 10mM salt solutions of Fe2(SO4)3 and Na2SO4, these solutions were actually prepared through the process of dilution in conical test tubes. 1mL of the 100mM salt solution for each substance was taken and diluted with 9mL of HPLC grade H2O to produce the 10mM salt solution of each substance.