User:Karlena L. Brown/Notebook/PVOH Research/2012/10/24: Difference between revisions
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==Notes== | ==Notes== | ||
* Originally Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub> was prepared at a 100mM concentration; however, this solution proved to be too concentrated for UV-Vis Analysis. The salt never fully dissolved into solution within the volumetric flask even with persistent heating. The solution remained very opaque and cloudy with precipitate falling out of solution. | |||
* The FeSO<sub>4</sub> 100mM solution was actually prepared at a later date which is why it was not previously recorded on the UV-Vis spectrum presented regarding the 944.5nM porphyrin sample. Also for the FeSO<sub>4</sub> salt solution, it was prepared in a 50mL volumetric flask instead of a 100mL volumetric flask. | |||
Revision as of 00:41, 24 November 2012
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OBJECTIVES
Porphyrin Salt Selection and PreparationsSO42- salts used:Fe2(SO4)3, Al3(SO4)3, FeSO4,& Na2SO4 Preparing 100mM Stock Solutions For Each Salt :
(Ensure that the volumetric calibration line is not surpassed or solution must be remade.) 100mM Salt Solution Calculations
MW Fe2(SO4)3: 278.91 g/mol 100mM Fe2(SO4)3 × (100mL H2O / 1000mL H2O) × (278.91g Fe2(SO4)3 / 1 mol Fe2(SO4)3) = 2.8791g Fe2(SO4)3
MW Al3(SO4)3: 356.37 g/mol 100mM Al3(SO4)3 × (100mL H2O / 1000mL H2O) × (656.37g Al3(SO4)3 / 1 mol Al3(SO4)3) = 6.5637g Al3(SO4)3
MW FeSO4: 278.01 g/mol 100mM FeSO4 × (50mL H2O / 1000mL H2O) × (278.01g FeSO4 / 1 mol FeSO4) = 1.3901g FeSO4
MW Na2SO4: 142.04 g/mol 100mM Na2SO4 × (100mL H2O / 1000mL H2O) × (142.04g Na2SO4 / 1 mol Na2SO4Na2SO4) = 1.4204g Na2SO4
UV-Vis Spectrum of Porphyrin with SaltsA specific porphyrin solution (944.5nM) was selected for exemplary UV - Vis analysis pertaining to each salt. Furthermore, 0.1mL each salt was combined with this particular porphyrin solution. Notes
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