User:Karlena L. Brown/Notebook/PVOH Research/2012/10/19: Difference between revisions
From OpenWetWare
(→Notes) |
|||
(6 intermediate revisions by the same user not shown) | |||
Line 38: | Line 38: | ||
==Atomic Absorption NaMT-CuSO4 Exchanged Filtrate Solutions== | ==Atomic Absorption NaMT-CuSO4 Exchanged Filtrate Solutions== | ||
'''RECALL ATOMIC ABSORPTION PROCEDURAL PROTOCOL 10/02/12''' | |||
'''Atomic Absorption Data and Calibration Curve Data from AA wizard:''' | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
Line 104: | Line 108: | ||
==Crosslinking Porphyrin Films== | ==Crosslinking Porphyrin Films== | ||
''' | '''RECALL PROTOCOL REGARDING CROSSLINKING GLUTALDEHYDE FILMS 9/21/12'''; however, pay attention to the two PVOH films that will not be placed in the fixant solution during the crosslinking procedure. | ||
{| {{table}} | {| {{table}} | ||
Line 126: | Line 130: | ||
* For PVOH 22K glutaldehyde porphyrin films that were crosslinked, all films maintained a swirled light pink / salmon color appearance. | * For PVOH 22K glutaldehyde porphyrin films that were crosslinked, all films maintained a swirled light pink / salmon color appearance. | ||
* During crosslinking and excessive heating, the porphyrin films began to turn lime green; most films turned green upon entering the fixant solution composed of Na<sub>2<sub>SO</sub>4</sub> and H<sub>2</sub>SO</sub>4</sub>. | * During crosslinking and excessive heating, the porphyrin films began to turn lime green; most films turned green upon entering the fixant solution composed of Na<sub>2<sub>SO</sub>4</sub> and H<sub>2</sub>SO</sub>4</sub>. | ||
* Most PVOH 22K glutaldehyde porphyrin films had some amount of porphyrin leach out into various solutions during crosslinking. For this reason, after every switch or movement of a porphyrin film to another solution, a small amount (10mL) of each solution was collected for UV-Vis analysis. | * However, the PVOH films in which two drops of HCl were added, initially started and appeared green before crosslinking. | ||
* Most PVOH 22K glutaldehyde porphyrin films had some amount of porphyrin leach out into various solutions during crosslinking. Many solutions maintained a yellow green / mantis green appearance after the removal of porphyrin films. | |||
* For this reason, after every switch or movement of a porphyrin film to another solution, a small amount (10mL) of each solution was collected for UV-Vis analysis. After crosslinking, all films were then stored in beakers full of distilled H<sub>2</sub>O. | |||
* Upon entering the distilled H<sub>2</sub>O, each film reverted back and turned pink in color again (darker pink on edges). | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 01:56, 24 November 2012
PVOH Research | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OBJECTIVES
New CuSO4 Dilution Standards for Atomic Absorption
(20 ppm CuSO4)V1 = (0.1 ppm CuSO4)(12mL) V1 = 0.6mL of 20 ppm CuSO4
(20 ppm CuSO4)V1 = (0.5 ppm CuSO4)(12mL) V1 = 1.2mL of 20 ppm CuSO4
(20 ppm CuSO4)V1 = (1 ppm CuSO4)(12mL) V1 = 3mL of 20 ppm CuSO4
(20 ppm CuSO4)V1 = (2 ppm CuSO4)(12mL) V1 = 6mL of 20 ppm CuSO4
(20 ppm CuSO4)V1 = (3 ppm CuSO4)(12mL) V1 = 9mL of 20 ppm CuSO4 Using the following concentrations(shown above), a calibration curve was prepared.
Atomic Absorption NaMT-CuSO4 Exchanged Filtrate SolutionsRECALL ATOMIC ABSORPTION PROCEDURAL PROTOCOL 10/02/12 Atomic Absorption Data and Calibration Curve Data from AA wizard:
Fixant Solution Preparation
(Actual Na2SO4 Mass: 199.9953g)
Crosslinking Porphyrin FilmsRECALL PROTOCOL REGARDING CROSSLINKING GLUTALDEHYDE FILMS 9/21/12; however, pay attention to the two PVOH films that will not be placed in the fixant solution during the crosslinking procedure.
Notes
|