User:Karlena L. Brown/Notebook/PVOH Research/2012/10/17 - Revision history

Karlena L. Brown: /* Porphyrin Dilution Calculations */

2012-11-30T20:43:34Z

Porphyrin Dilution Calculations

←Older revision		Revision as of 20:43, 30 November 2012
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	==Porphyrin Dilution Calculations==		==Porphyrin Dilution Calculations==

-	'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED'''	+	'''STOCK SOLUTION PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED'''
-		+

	* '''Solution of 9.445 μM porphyrin'''		* '''Solution of 9.445 μM porphyrin'''

Karlena L. Brown: /* Porphyrin Dilution Calculations */

2012-11-30T20:43:17Z

Porphyrin Dilution Calculations

←Older revision		Revision as of 20:43, 30 November 2012
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	'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED'''		'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED'''
		+

	* '''Solution of 9.445 μM porphyrin'''		* '''Solution of 9.445 μM porphyrin'''

Karlena L. Brown: /* Porphyrin Dilution Calculations */

2012-11-30T20:40:32Z

Porphyrin Dilution Calculations

←Older revision		Revision as of 20:40, 30 November 2012
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	==Porphyrin Dilution Calculations==		==Porphyrin Dilution Calculations==

-	~~ORIGINAL~~ STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED ~~BELOW~~	+	'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED'''

	* '''Solution of 9.445 μM porphyrin'''		* '''Solution of 9.445 μM porphyrin'''

Karlena L. Brown: /* Porphyrin Dilution Calculations */

2012-11-30T20:39:56Z

Porphyrin Dilution Calculations

←Older revision		Revision as of 20:39, 30 November 2012
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	==Porphyrin Dilution Calculations==		==Porphyrin Dilution Calculations==
		+
		+	ORIGINAL STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED BELOW

	* '''Solution of 9.445 μM porphyrin'''		* '''Solution of 9.445 μM porphyrin'''

Karlena L. Brown: /* Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures */

2012-11-24T08:51:19Z

Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures

←Older revision		Revision as of 08:51, 24 November 2012
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	==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures==		==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures==
-	RECALL PROCEDURAL PROTOCOL FOR Cu<sup>2+</sup> FILM EXCHANGED SOLUTIONS & VACUUM FILTRATION PROCEDURES 10/05/12; however, instead of using clay, use ~ 1g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.	+	'''RECALL PROCEDURAL PROTOCOL FOR Cu<sup>2+</sup> FILM EXCHANGED SOLUTIONS & VACUUM FILTRATION PROCEDURES 10/05/12'''; however, instead of using clay, use ~ 1g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.

	{\| {{table}}		{\| {{table}}

Karlena L. Brown: /* Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures */

2012-11-24T08:48:37Z

Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures

←Older revision		Revision as of 08:48, 24 November 2012
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	==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures==		==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures==
-	~~'''Follow procedural protocol for~~ Cu<sup>2+</sup> ~~film exchanged solutions~~ & ~~vacuum filtration procedures~~ 10/05/12; however, instead of using clay, use ~~1.0g~~ films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.~~'''~~	+	RECALL PROCEDURAL PROTOCOL FOR Cu<sup>2+</sup> FILM EXCHANGED SOLUTIONS & VACUUM FILTRATION PROCEDURES 10/05/12; however, instead of using clay, use ~ 1g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.

	{\| {{table}}		{\| {{table}}

Karlena L. Brown: /* Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures */

2012-11-24T04:35:03Z

Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration Procedures

←Older revision		Revision as of 04:35, 24 November 2012
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	\| align="center" style="background:#f0f0f0;"\|'''Films Prepared'''		\| align="center" style="background:#f0f0f0;"\|'''Films Prepared'''
	\| align="center" style="background:#f0f0f0;"\|'''Original Film Mass (g)'''		\| align="center" style="background:#f0f0f0;"\|'''Original Film Mass (g)'''
-	\| align="center" style="background:#f0f0f0;"\|'''Film Mass After ~~10min~~ Exchange (g)'''	+	\| align="center" style="background:#f0f0f0;"\|'''Film Mass After 10 min Exchange (g)'''
	\|-		\|-
	\| PVOH 22K \|\|0.9000\|\|0.7582		\| PVOH 22K \|\|0.9000\|\|0.7582

Karlena L. Brown: /* Porphyrin Film Preparations */

2012-11-24T04:34:40Z

Porphyrin Film Preparations

←Older revision		Revision as of 04:34, 24 November 2012
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	==Porphyrin Film Preparations==		==Porphyrin Film Preparations==
-	'''Follow procedural protocol for preparing glutaldehyde films 9/25/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde to each film before pouring substance into a Teflon dish. Also for two PVOH films, be sure to add two drops of HCl to observe if the addition of the acid aids in securing all of the porphyrin within the films.'''	+	'''Follow procedural protocol for preparing glutaldehyde films 9/19/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde to each film before pouring substance into a Teflon dish. Also for two PVOH films, be sure to add two drops of HCl to observe if the addition of the acid aids in securing all of the porphyrin within the films.'''

	{\| {{table}}		{\| {{table}}

Karlena L. Brown: /* Porphyrin Film Preparations */

2012-11-24T04:27:16Z

Porphyrin Film Preparations

←Older revision		Revision as of 04:27, 24 November 2012
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	==Porphyrin Film Preparations==		==Porphyrin Film Preparations==
-	'''Follow procedural protocol for preparing glutaldehyde films 9/25/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde ~~for~~ each film before pouring substance into a Teflon dish.'''	+	'''Follow procedural protocol for preparing glutaldehyde films 9/25/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde to each film before pouring substance into a Teflon dish. Also for two PVOH films, be sure to add two drops of HCl to observe if the addition of the acid aids in securing all of the porphyrin within the films.'''

	{\| {{table}}		{\| {{table}}
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	\| align="center" style="background:#f0f0f0;"\|'''Glutaldehyde Amount (mL)'''		\| align="center" style="background:#f0f0f0;"\|'''Glutaldehyde Amount (mL)'''
	\| align="center" style="background:#f0f0f0;"\|'''Porphyrin Amount (mL)'''		\| align="center" style="background:#f0f0f0;"\|'''Porphyrin Amount (mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''HCl Addition (Y/N)'''
	\|-		\|-
-	\| PVOH 22K w/ GA & PP(g)\|\|1.0073\|\|1.0\|\|1.0	+	\| PVOH 22K w/ GA & PP(g)\|\|1.0073\|\|1.0\|\|1.0\|\|N
	\|-		\|-
-	\| PVOH 22K w/ GA & PP(g)\|\|1.0067\|\|1.0\|\|1.0	+	\| PVOH 22K w/ GA & PP(g)\|\|1.0067\|\|1.0\|\|1.0\|\|Y
	\|-		\|-
-	\| PVOH 22K w/ GA & PP(g)\|\|1.0523\|\|0.5\|\|1.0	+	\| PVOH 22K w/ GA & PP(g)\|\|1.0523\|\|0.5\|\|1.0\|\|N
	\|-		\|-
-	\| PVOH 22K w/ GA & PP(g)\|\|1.0211\|\|0.5\|\|1.0	+	\| PVOH 22K w/ GA & PP(g)\|\|1.0211\|\|0.5\|\|1.0\|\|Y
	\|}		\|}

	__NOTOC__		__NOTOC__

Karlena L. Brown: /* Porphyrin Dilution Calculations */

2012-11-24T03:50:24Z

Porphyrin Dilution Calculations

←Older revision		Revision as of 04:35, 24 November 2012
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	\| align="center" style="background:#f0f0f0;"\|'''Films Prepared'''		\| align="center" style="background:#f0f0f0;"\|'''Films Prepared'''
	\| align="center" style="background:#f0f0f0;"\|'''Original Film Mass (g)'''		\| align="center" style="background:#f0f0f0;"\|'''Original Film Mass (g)'''
-	\| align="center" style="background:#f0f0f0;"\|'''Film Mass After ~~10min~~ Exchange (g)'''	+	\| align="center" style="background:#f0f0f0;"\|'''Film Mass After 10 min Exchange (g)'''
	\|-		\|-
	\| PVOH 22K \|\|0.9000\|\|0.7582		\| PVOH 22K \|\|0.9000\|\|0.7582

←Older revision		Revision as of 03:50, 24 November 2012
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	==Porphyrin Dilution Calculations==		==Porphyrin Dilution Calculations==

-	* '''Solution of 94.45 μM porphyrin'''	+	* '''Solution of 9.445 μM porphyrin'''
	(94.45 μM)(V<sub>1</sub>)=(9.445 μM)(10mL)		(94.45 μM)(V<sub>1</sub>)=(9.445 μM)(10mL)
	V<sub>1</sub> = 1mL of 94.45 μM porphyrin		V<sub>1</sub> = 1mL of 94.45 μM porphyrin

-	* '''Solution of 9.~~445 μM~~ porphyrin'''	+	* '''Solution of 944.5 nM porphyrin'''
	(9.445 μM)(V<sub>1</sub>)=(944.5 nM)(10mL)		(9.445 μM)(V<sub>1</sub>)=(944.5 nM)(10mL)
	V<sub>1</sub> = 1mL of 9.445 μM porphyrin		V<sub>1</sub> = 1mL of 9.445 μM porphyrin

-	* '''Solution of ~~944~~.5 nM porphyrin'''	+	* '''Solution of 94.45 nM porphyrin'''
	(944.5 nM)(V<sub>1</sub>)=(94.45 nM)(10mL)		(944.5 nM)(V<sub>1</sub>)=(94.45 nM)(10mL)
	V<sub>1</sub> = 1mL of 944.5 nM porphyrin		V<sub>1</sub> = 1mL of 944.5 nM porphyrin

-	* '''Solution of ~~944~~.5 nM porphyrin'''	+	* '''Solution of 9.445 nM porphyrin'''
	(944.5 nM)(V<sub>1</sub>)=(9.445 nM)(10mL)		(944.5 nM)(V<sub>1</sub>)=(9.445 nM)(10mL)
	V<sub>1</sub> = 0.1mL of 944.5 nM porphyrin		V<sub>1</sub> = 0.1mL of 944.5 nM porphyrin

-	* '''Solution of 94.45 μM porphyrin'''	+	* '''Solution of 1.89 μM porphyrin'''
	(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)		(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
	V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin		V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin

-	* '''Solution of 94.45 uM porphyrin'''	+	* '''Solution of 3.78 uM porphyrin'''
	(94.45 μM)(V<sub>1</sub>)=(3.78 μM)(10mL)		(94.45 μM)(V<sub>1</sub>)=(3.78 μM)(10mL)
	V<sub>1</sub> = 0.4mL of 94.45 μM porphyrin		V<sub>1</sub> = 0.4mL of 94.45 μM porphyrin