User:Karlena L. Brown/Notebook/PVOH Research/2012/10/17: Difference between revisions
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==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures== | ==Preparations For Cu<sup>2+</sup> Film Exchanged Solutions & Vacuum Filtration Procedures== | ||
''' | '''RECALL PROCEDURAL PROTOCOL FOR Cu<sup>2+</sup> FILM EXCHANGED SOLUTIONS & VACUUM FILTRATION PROCEDURES 10/05/12'''; however, instead of using clay, use ~ 1g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval. | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''Films Prepared''' | | align="center" style="background:#f0f0f0;"|'''Films Prepared''' | ||
| align="center" style="background:#f0f0f0;"|'''Original Film Mass (g)''' | | align="center" style="background:#f0f0f0;"|'''Original Film Mass (g)''' | ||
| align="center" style="background:#f0f0f0;"|'''Film Mass After | | align="center" style="background:#f0f0f0;"|'''Film Mass After 10 min Exchange (g)''' | ||
|- | |- | ||
| PVOH 22K ||0.9000||0.7582 | | PVOH 22K ||0.9000||0.7582 | ||
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==Porphyrin Dilution Calculations== | ==Porphyrin Dilution Calculations== | ||
'''STOCK SOLUTION PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED''' | |||
* '''Solution of 9.445 μM porphyrin''' | * '''Solution of 9.445 μM porphyrin''' | ||
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==Porphyrin Film Preparations== | ==Porphyrin Film Preparations== | ||
'''Follow procedural protocol for preparing glutaldehyde films 9/ | '''Follow procedural protocol for preparing glutaldehyde films 9/19/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde to each film before pouring substance into a Teflon dish. Also for two PVOH films, be sure to add two drops of HCl to observe if the addition of the acid aids in securing all of the porphyrin within the films.''' | ||
{| {{table}} | {| {{table}} | ||
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| align="center" style="background:#f0f0f0;"|'''Glutaldehyde Amount (mL)''' | | align="center" style="background:#f0f0f0;"|'''Glutaldehyde Amount (mL)''' | ||
| align="center" style="background:#f0f0f0;"|'''Porphyrin Amount (mL)''' | | align="center" style="background:#f0f0f0;"|'''Porphyrin Amount (mL)''' | ||
| align="center" style="background:#f0f0f0;"|'''HCl Addition (Y/N)''' | |||
|- | |- | ||
| PVOH 22K w/ GA & PP(g)||1.0073||1.0||1.0 | | PVOH 22K w/ GA & PP(g)||1.0073||1.0||1.0||N | ||
|- | |- | ||
| PVOH 22K w/ GA & PP(g)||1.0067||1.0||1.0 | | PVOH 22K w/ GA & PP(g)||1.0067||1.0||1.0||Y | ||
|- | |- | ||
| PVOH 22K w/ GA & PP(g)||1.0523||0.5||1.0 | | PVOH 22K w/ GA & PP(g)||1.0523||0.5||1.0||N | ||
|- | |- | ||
| PVOH 22K w/ GA & PP(g)||1.0211||0.5||1.0 | | PVOH 22K w/ GA & PP(g)||1.0211||0.5||1.0||Y | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 13:43, 30 November 2012
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OBJECTIVES
Preparations For Cu2+ Film Exchanged Solutions & Vacuum Filtration ProceduresRECALL PROCEDURAL PROTOCOL FOR Cu2+ FILM EXCHANGED SOLUTIONS & VACUUM FILTRATION PROCEDURES 10/05/12; however, instead of using clay, use ~ 1g films prepared containing PVOH, glutaldehyde, and clay samples. In addition, only exchange films for solely 10min time interval.
UV - Vis Procedures
Porphyrin Dilution CalculationsSTOCK SOLUTION PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATIONS LISTED
(94.45 μM)(V1)=(9.445 μM)(10mL) V1 = 1mL of 94.45 μM porphyrin
(9.445 μM)(V1)=(944.5 nM)(10mL) V1 = 1mL of 9.445 μM porphyrin
(944.5 nM)(V1)=(94.45 nM)(10mL) V1 = 1mL of 944.5 nM porphyrin
(944.5 nM)(V1)=(9.445 nM)(10mL) V1 = 0.1mL of 944.5 nM porphyrin
(94.45 μM)(V1)=(1.89 μM)(10mL) V1 = 0.2mL of 94.45 μM porphyrin
(94.45 μM)(V1)=(3.78 μM)(10mL) V1 = 0.4mL of 94.45 μM porphyrin UV-Vis of Porphyrin Solutions
* Wavelength range = 200 - 800nm * Slit width = 1.0nm UV-Vis Analysis Notes
Porphyrin Film PreparationsFollow procedural protocol for preparing glutaldehyde films 9/19/12; however, add ~ 1mL of porphyrin in addition to glutaldehyde to each film before pouring substance into a Teflon dish. Also for two PVOH films, be sure to add two drops of HCl to observe if the addition of the acid aids in securing all of the porphyrin within the films.
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