OBJECTIVES
 Crosslink all glutaldehyde films
 Make standard dilution CuSO_{4} solutions from the 20ppm CuSO_{4} stock solution for atomic absorption
 Run absorption on all CuSO_{4} dilution samples prepared
Crosslinking Glutaldehyde Films
PROCEDURAL STEPS FOR CROSSLINKING CAN BE FOUND UNDER 9/21/12
Prepared PVOH 22K Films For Crosslinking
'
 PVOH 22K w/ GA
 PVOH 22K w/ GA & PDMS
 PVOH 22K w/ GA & NaMT
 PVOH 22K w/ GA & 100% CEC NaMT
 PVOH 22K w/ GA & 50% CEC NaMT

Original Mass PVOH used (g)  0.5141  0.9499  0.4880  0.7665  0.4691

New Crosslinked Mass PVOH (g)  0.5126  0.8521  0.9621  1.3971  0.9567

Atomic Absorption Procedures
 Initiate the copper lamp by rotating the knob on the right side of the machine to the copper lamp
 Flip slit width to 0.7nm by rotating the knob on the left
 Place distilled H_{2}O in the sample entry tube
 Turn on the machine, acetylene gas(red tank), and the air tank(yellow tank)
 Open AA wizard on the computer
 Click element select, flame, and the edit parameters window
 Select a metal (Cu with wavelength = 354.8nm)
 Edit measurement parameters to SMMM and then edit calibration curve to ppm and first order
 Select next and double click on calibration curve
 Enter calibration and number of standard solutions created: lowest concentration to highest concentration
 Next, label all samples and hit "Collect Send"
 Once the Instrument Checklist box appears check off all boxes
 Hold the purge button on the machine to check that the air and fuel level are at the right level(1,8,8)
 Click line search and then finish.
 Hold the purge and ignite button on the AA until a flame appears
 Allow the sample tube to collect distilled H_{2}O for an autozero
 After autozero is complete, sample labeled samples in the order in which they were entered
Atomic Absorption Standard Dilutions & Calculations
Na_{2}SO_{4} Dilution Standards
12mL 1 ppm Na_{2}SO_{4} requires:
(20ppm Na_{2}SO_{4})V_{1} = (1ppm Na_{2}SO_{4})(12mL)
V_{1} = 0.6mL
12mL 2 ppm Na_{2}SO_{4} requires:
(20ppm Na_{2}SO_{4})V_{1} = (2ppm Na_{2}SO_{4})(12mL)
V_{1} = 1.2mL
12mL 5 ppm Na_{2}SO_{4} requires:
(20ppm Na_{2}SO_{4})V_{1} = (5ppm Na_{2}SO_{4})(12mL)
V_{1} = 3mL
12mL 10 ppm Na_{2}SO_{4} requires:
(20ppm Na_{2}SO_{4})V_{1} = (10ppm Na_{2}SO_{4})(12mL)
V_{1} = 6mL
12mL 15 ppm Na_{2}SO_{4} requires:
(20ppm Na_{2}SO_{4})V_{1} = (15ppm Na_{2}SO_{4})(12mL)
V_{1} = 9mL
CuSO_{4} Dilution Standards
12mL 1 ppm CuSO_{4} requires:
(20ppm CuSO_{4})V_{1} = (1ppm CuSO_{4})(12mL)
V_{1} = 0.6mL
12mL 2 ppm CuSO_{4} requires:
(20ppm CuSO_{4})V_{1} = (2ppm CuSO_{4})(12mL)
V_{1} = 1.2mL
12mL 5 ppm CuSO_{4} requires:
(20ppm CuSO_{4})V_{1} = (5ppm CuSO_{4})(12mL)
V_{1} = 3mL
12mL 10 ppm CuSO_{4} requires:
(20ppm CuSO_{4})V_{1} = (10ppm CuSO_{4})(12mL)
V_{1} = 6mL
12mL 15 ppm CuSO_{4} requires:
(20ppm CuSO_{4})V_{1} = (15ppm CuSO_{4})(12mL)
V_{1} = 9mL
Notes
 Dilution standards listed above for both Na_{2}SO_{4} and CuSO_{4} are incorrect and inaccurate due to miscalculations of stock solution concentrations
CuSO_{4} stock solution = 13.2 ppm
Na_{2}SO_{4} solution = 9.61 ppm
 For SMMM, typically the machine makes a single run, but takes an average absorbance from three sample collections
 Fuel gas parameter should always be at 1.8 L/min and support gas at 8 L/min
 Due gold nanoparticles clogging the collection tube for atomic absorption samples, all collection data was viewed as being inaccurate along with invalid. Therefore, the collection tube will be replaced and atomic absorption data will be collected next class.
