User:Kalkao/notebook/Freezing Away a Cell Stock
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< User:Kalkao | notebook
- Obtain 40% glycerol from lab supply room
- Obtain overnight suspension from 37*C incubation room
- Turn off the rotor to take out test tube cultures
- Restart the rotor at a speed between 7 and 8
- Obtain one cryogenic vial for each stock
- Add 1mL 40% glycerol and 1 mL culture to each cryogenic vial
- Use the flameboy to sterilize the pipette before extracting from glycerol or test tube
- Flame the opening of the glycerol bottle before and after each use
- Flame the opening of the test tube before and after each use
- Place the cryogenic vials in a freezer box
- Label the freezer box and place in -80*C storage