User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/18: Difference between revisions

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[[Image:PCR.BB0015.JPG|120px]]




Revision as of 10:44, 19 August 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • PCR of B0015.
  • PCR of pSB1T3.
  • See the in Gel.

Working

PCR of B0015

The point of this PCR is get double terminator and insert it into pSB1T3 backbone.

  • Amplified with preffix and suffix primers.


Reactives μL
Buffer Taq Standard 10X 5
dNTPs 8mM 1.25
Oligo F/R 2.5/2.5
DNA B0015 1
H2O 36.75
Taq DNA 1
Total 50μL



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