User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/02: Difference between revisions

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I left reaction overnight.
I left reaction overnight.
 
-Vector and -Insert are my controls to see right results.
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Revision as of 23:55, 3 August 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Ligation of Plasmid pSB1AK3/BBa_B0015 and Prom+Kanamycin.

Ligation

5:2 4:3 -Vector -Insert
DNA insert 5 4 5 0
DNA vector 2 3 0 3
Buffer T4 DNA Ligase NEB 1 1 1 1
T4 DNA Ligase NEB 1 1 0 0
H2O 1 1 4 6

I left reaction overnight. -Vector and -Insert are my controls to see right results.


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