User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/28: Difference between revisions

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(Autocreate 2011/07/28 Entry for User:Jose_Fabricio_Lopez/Notebook/Logbook)
 
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==Entry title==
=Objectives=
* Insert content here...
* Plasmid extraction of pUX19XKR5.
* Transformation of [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
* Restriction of today´s plasmid extraction.


==Transformation==
*Miguel's lab method.
==Plasmid extraction==
*Miguel's lab miniprep.
[[[[[InsertGel]]]]]
==Restriction==
*Restriction performed with SmaI, unique site in plasmid for [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/06|Extraction 1]] ,[[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/12|Extraction 2]] and Today´s extraction.
{| border=1
!
![[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/06|Extraction 1]]
![[User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/12|Extraction 2]]
!Today´s
|--
|Buffer4
|2μL
|2μL
|2μL
|--
|SmaI
|1μL
|1μL
|1μL
|--
|DNA
|10μL
|10μL
|10μL
|--
|H<sub>2</sub>O
|7μL
|7μL
|7μL
|--
|Total
|20μL
|20μL
|20μL
|}
I expect 1 band in cut DNA, I will choose for PCR the right plasmid and cleanest.


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__NOTOC__
__NOTOC__

Revision as of 00:16, 28 July 2011

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Objectives

  • Plasmid extraction of pUX19XKR5.
  • Transformation of BBa_B0015
  • Restriction of today´s plasmid extraction.

Transformation

  • Miguel's lab method.

Plasmid extraction

  • Miguel's lab miniprep.

[[[[[InsertGel]]]]]

Restriction


Extraction 1 Extraction 2 Today´s
Buffer4 2μL 2μL 2μL
SmaI 1μL 1μL 1μL
DNA 10μL 10μL 10μL
H2O 7μL 7μL 7μL
Total 20μL 20μL 20μL

I expect 1 band in cut DNA, I will choose for PCR the right plasmid and cleanest.


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