User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/27: Difference between revisions

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=Objectives=
=Objectives=
* Plasmid extraction from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/26
* Plasmid extraction from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/26|yesterday cultures]]  
yesterday cultures]]  
* Restriction of [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/22|Purified PCR]].
* Restriction of [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/22|Purified PCR]].
* See in a gel extraction.
* See in a gel extraction.

Revision as of 11:17, 28 June 2011

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Objectives

Plasmid extraction

  • Performed with LB-liquid mediums from yesterday.
  • I used Miguel´s Lab miniprep.
  • Final dilution in 50μL

Restriction

  • In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI.
Reactives Dilutions
Buffer4 10x NEB 1μL
BSA 1μL
PCR Purf. 12μL
H2O 4 μL
ApaI 1μL
SacI 1μL