User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/27: Difference between revisions
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*In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI. | *In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI. | ||
{ | {| border=1 | ||
} | !Reactives | ||
!Dilutions | |||
|-- | |||
|Buffer4 10x NEB | |||
| 1μL | |||
|-- | |||
|BSA | |||
| 1μL | |||
|-- | |||
| PCR Purf. | |||
| 12μL | |||
|-- | |||
|H<sub>2</sub>O | |||
| 4 μL | |||
|-- | |||
| ApaI | |||
| 1μL | |||
|-- | |||
| SacI | |||
| 1μL | |||
|} | |||
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Revision as of 11:16, 28 June 2011
 iGEM 2011
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Objectives
yesterday cultures]]
Plasmid extraction
Restriction
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