User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/27: Difference between revisions

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* See in a gel extraction.
* See in a gel extraction.


==Plasmid extraction===
==Plasmid extraction==
*Performed with LB-liquid mediums from yesterday.
*Performed with LB-liquid mediums from yesterday.
*I used Miguel´s Lab miniprep.
*I used Miguel´s Lab miniprep.

Revision as of 11:11, 28 June 2011

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Objectives

  • Plasmid extraction from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/26

yesterday cultures]]

Plasmid extraction

  • Performed with LB-liquid mediums from yesterday.
  • I used Miguel´s Lab miniprep.
  • Final dilution in 50μL

Restriction

  • In order to standardize pBBRmc-S5 I will cut PCR Purification with SacI & ApaI.

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