User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/20: Difference between revisions
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=Objectives= | =Objectives= | ||
*PCR from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/19|extractions]] | *PCR from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/19|extractions]] | ||
*Primers (5 pM/μL) (new set because we are improving new protocol of standardization). | |||
**Primer Forward= CGG GCC CCA TTA TTA TCA TGA CAT TAA CCT ATA AAA ATA GG | |||
**Primer Reverse= CGA GCT CGC CTT TTG CTC ACA TCT TCT TTC C | |||
==PCR== | ==PCR== | ||
==PCR amplification== | *DNA was obtained from [[User:Jose Fabricio Lopez/Notebook/Logbook/2011/06/19|extractions]] and diluted(2μL DNA in 98μL H<sub>2</sub>O) | ||
===PCR amplification=== | |||
{| border="1" | {| border="1" | ||
! Reactives | ! Reactives | ||
Line 56: | Line 60: | ||
| 50μL | | 50μL | ||
| 50μL | | 50μL | ||
|} | |||
==Control PCR== | |||
{| border="1" | |||
! Reactives | |||
! -R | |||
! -F | |||
! -O | |||
|-- | |||
|Buffer Standard Taq 10x NEB | |||
| 2μL | |||
| 2μL | |||
| 2μL | |||
|-- | |||
| Primer F | |||
| 2μL | |||
| 0μL | |||
| 0μL | |||
|-- | |||
| Primer R | |||
| 0μL | |||
| 2μL | |||
| 0μL | |||
|-- | |||
| .4mM dNTPs | |||
| 10μL | |||
| 10μL | |||
| 10μL | |||
|-- | |||
| DNA(Pablo's extraction) | |||
| 2μL | |||
| 2μL | |||
| 2μL | |||
|-- | |||
| Taq | |||
| .2μL | |||
| .2μL | |||
| .2μL | |||
|-- | |||
| H<sub>2</sub>O | |||
| 3.8 | |||
| 3.8 | |||
| 3.8 | |||
|-- | |||
| Total | |||
| 20μL | |||
| 20μL | |||
| 20μL | |||
|} | |} | ||
Revision as of 13:16, 21 June 2011
iGEM 2011 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Objectives
PCR
PCR amplification
Control PCR
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