User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/29: Difference between revisions

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‡[[User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28 |Fabricio´s extraction]]
‡[[User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28 |Fabricio´s extraction]]
[[Image:IMG 0823.JPG|450px]]




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Revision as of 17:28, 29 May 2011

iGEM 2011 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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WetLab

  • Purification of digestion's products.
    • I left over night digestion, trying to get the best digestion as possible.
    • Inactivated enzymes at 65°C, 20 minutes.
    • High Pure PCR Product Purification Kit.
  • Electrophoresis gel of digestion
1 2 3 4 5 6 7 8
Ladder 1Kb 5μL ‡ 5μL † 10μL † 10μL ‡ Control of Digestion pBBRmcS5† pBBRmcS5‡


Daniel´s extraction

Fabricio´s extraction