User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/01
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Thursday 1st July 2010I performed 3 digestions of the 3 plasmids for posterior ligations as follows:
Reactive..........1x [ul]
Reactive..........1x [ul]
Reactive........1x [ul] I also did a hot start PCR (with rtth polymerase) to introduce the trpLp promoter into a medium copy number plasmid with a reverse primer containing the promoter primed at the prefix and a forward one primed at the suffix.
Reactive.........................1x...............7.x
Reactive...........1x (ul)........7x (ul)
|