User:Jonathan Cline/Notebook/Melaminometer/Technical Summary
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Technical Summary of Melamine Detector
Melamine can be metabolized by biochemical/enzymatic process in bacteria and/or other compatible organisms. Using recombinant DNA techniques, plasmids containing genes encoding specific enzymes will be used to modify the target organism (aka: the chassis). There may be one to four enzymes required for metabolism to desired products.
Complete metabolic pathway:
melamine --(1)--> ammeline --(2)--> ammelide --(3)--> cyanuric acid cyanuric acid --(4)--> biuret --(5)--> allophante --(6)--> 2 NH3 + some CO2 * (1): melamine deaminase:triA --^ NH4+ + H2O * (2): ammeline aminohydralase:triA --^ NH4+ + H2O * (3): ammelide aminohydralase:trzC --^ NH4+ + H2O * (4): atzD/trzD --^ NH4+ + H2O * (5): atzE * (6): atzF
Desired logical operation:
if [ melamine (AND) cyanuric acid ] then amplify express GFP ; for example stop else repress GFP ; for example stop endif