User:Jonathan Cline/Notebook/Melaminometer/Technical Summary: Difference between revisions
From OpenWetWare
No edit summary |
|||
| Line 8: | Line 8: | ||
<pre> | <pre> | ||
melamine --(1)--> ammeline --(2)--> ammelide --(3)--> cyanuric acid --(4)--> biuret --(5)--> allophante --(6)--> 2 NH3 + some CO2 | melamine --(1)--> ammeline --(2)--> ammelide --(3)--> cyanuric acid | ||
cyanuric acid --(4)--> biuret --(5)--> allophante --(6)--> 2 NH3 + some CO2 | |||
Revision as of 01:33, 16 October 2008
Technical Summary of Melamine Detector
Melamine can be metabolized by biochemical/enzymatic process in bacteria and/or other compatible organisms. Using recombinant DNA techniques, plasmids containing genes encoding specific enzymes will be used to modify the target organism (aka: the chassis). There may be one to four enzymes required for metabolism to desired products.
Complete metabolic pathway:
melamine --(1)--> ammeline --(2)--> ammelide --(3)--> cyanuric acid cyanuric acid --(4)--> biuret --(5)--> allophante --(6)--> 2 NH3 + some CO2 * (1): melamine deaminase:triA --^ NH4+ + H2O * (2): ammeline aminohydralase:triA --^ NH4+ + H2O * (3): ammelide aminohydralase:trzC --^ NH4+ + H2O * (4): atzD/trzD --^ NH4+ + H2O * (5): atzE * (6): atzF
Desired logical operation:
if [ melamine (AND) cyanuric acid ] then amplify express GFP ; for example stop else repress GFP ; for example stop endif
