User:Jonathan Cline/Notebook/Melaminometer/Meetings
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Abbreviations:
- JC = jcline
- NYMU = National Yang Ming University, Taipei, Taiwan
- Chuan-Hsiung Chang's lab
- Chih-Hsien Yang OWW User:Blent
- MP = Meredith L. Patterson
2008-11-04 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- Introductions
- Capabilities, existing NYMU technology & licensing
- product goal discussion
- OWW open source project
- set next meeting date/time 2008-11-18
2008-11-11 JC+SG
- Discussion of competitive methods for detection.
- ELISA
- You make a serial dilution and then get a good estimate of your antigen concentration by identifying the dilution at which you can first identify a color change.
2008-11-18 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- postponed due to travel.
- set next meeting date/time 2008-11-25
2008-11-25 JC+NYMU
Attendees:
- JC
- NYMU
Conf call Summary
- Introductions
- NYMU team assignments
- Discuss NYMU presentation & JC OWW research
- Custom protein
- Example, combine light sensing protein in cyanobacteria with melamine receptor for detection
- Chassis
- Idea, use ADP1 as it has been proposed before
- Custom protein
- Set next steps:
- NYMU Want to get current market ELISA kit for evaluation & calibration in lab
- Need to scope where to get other materials
- NYMU Need to scope designing enzymes into e.coli
- JC to investigate creating biobricks from known seqs in research
- Need to investigate noise issues for detection in sample
- Need to investigate regulating enzymes
- Set next meeting date/time 2008-12-04 9AM (TAIPEI time)
JC Wiki Updates
- Created rough outline schedule on wiki
- created meeting notes on wiki
2008-12-4 JC+NYMU
Attendees:
- JC
- NYMU
- Chih-Hsien Yang
Conf call Summary
- Introductions
- Discuss team work method
- All data will be on wiki.
- No secondary mailing list or doc sharing site at this time. All docs uploaded to wiki.
- Discuss Schedule
- Discuss current work
- ELISA kit study
- Assumed to be more costly than synbio solution, since kit needs purified enzymes in the kit
- Melaminometer must be better performance than ELISA kit to be successful
- Enzymatic method study
- Contradiction between biocyc.org and papers and multiple action from triA
- Reproduce previous experimental result from 2001 paper; verify triA activity
- NYMU will request materials from author
- Antibody method study
- Need to model this for melamine; find homologues
- 12227, 12228 from 2001-paper.
- May be more successful method than pH detection
- Single plasmid should be possible for the multiple genes; will make fabrication easier
- ELISA kit study
- Next week's work
- JC do patent search for related products
- NYMU investigate ELISA kit; two examples:
- NYMU define materials required for testing (positive, negative controls)
- NYMU compare homologues of melamine deaminase
- MP compare homologues of enzymes
- NYMU contact author of 2001 for strain availability
2008-12-11 JC+NYMU+MP
- alternative methods (in vivo and in vitro) - CHC
- ELISA
- MEMS (e.g. electronic nose, lab-on-chip)
- synthetic biology
- or combinations
- agenda next week
- discussion of designs
- assessment and evaluation of detection methods
- make a action plan before the end of Dec. 2008
2008-12-18 JC+NYMU+MP
- Suggestion to recognize physical structure of melamine
- size
- charge
- hydrophobicity
- Need to duplicate same design method for cyanuric acid too
- Need to convert thresholds from mg to molar depending on sample
- Using ph sensor - cells may balance ph leading to detection problems
- Discussion of using multiple methods together
- JC suggests NYMU call TW food testing agencies to get their opinion on if/when/how they plan to use ELISA testing - none of the agencies are listed as using ELISA; why?
- Next mtg set for Dec 25 9am-TW for JC+Blent
- Next mtg set for Dec 31 9am-TW for JC+NYMU
2008-12-25 JC+Blent
- Ordering of pure melamine sample:
- Sigma CAS number: 108-78-1 (1,400 NTD)
- Evaluation of pH sensor in higher pH condition
2008-12-30 JC+blent
- skipped
2009-01-08 JC+NYMU
- idea #1
- idea #2
- modeling and preliminary experiments
- sensitivity
- specificity
- reaction time
2009-01-15 JC+NYMU
- where is the market?
- to have marketing and social impact
- shorter reaction time (less than 20 min)
- better sensitivity (outcompete with ELISA)
- to have academic impact
- novel and new method