User:Jonathan Cline/Notebook/Melaminometer

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Contents

Melaminometer: Easily Detect Harmful Melamine and Related Analogues.

Monday, October 27, 2008 - China discovers eggs tainted with melamine. By DAVID BARBOZA, The New York Times. SHANGHAI, China - Hong Kong food inspectors have found eggs imported from northeast China to be contaminated with high levels of melamine, the toxic industrial additive at the heart of an adulteration scandal over Chinese milk products. The findings have raised new concerns here that a far wider array of China-produced foods than previously believed could be contaminated by melamine, which already has sickened more than 50,000 children in China and led to at least four deaths.

150px-Melamine.svg.png Melamine C3H6N6


For biologists: Read the "Much needed product for world health" proposal first, then skip to the "Specific Metabolic Activity".

For DIY-biologists or iGEM types: Read the "As proof of DIY Bio" proposal first, then skip to "Biochemistry Background".

For non-scientists and/or normal people: Read the "Much needed product for world health" proposal first, then skip to "Toxicology Background" and other background information.


Project Proposal

Product Need

  • Commercial food testing is unlikely to change in the short term
  • Commercial testing has failed to meet safety standards demanded by consumers
  • A method for establishing harmful content in food is needed at "Point-of-care"
    • Harmful product may especially exist in resource-constrained communities where commercial testing is lax
    • Consumers should have a means of testing food themselves.

Background

Technical

Modeling

Historical Perspective

November 2008: U.S. FDA halts all dairy imports from China, plus cheese, cereals, bakery products, ready-to-eat packaged breakfast foods, soft drinks, soft candy, cat and dog food.

[DETENTION WITHOUT PHYSICAL EXAMINATION OF ALL MILK PRODUCTS, MILK DERIVED INGREDIENTS AND FINISHED FOOD PRODUCTS CONTAINING MILK FROM CHINA DUE TO THE PRESENCE OF MELAMINE AND/OR MELAMINE ANALOGS]

October 2008: Harmful levels of Melamine contamination found in eggs exported from China to Hong Kong, in bread in Taiwan, and in crackers in Philippines. Popular media reports over 50,000 people adversely affected.

2008: Large-scale contamination found in wide variety of milk products exported from China. Hospitalized and/or affected population reported in popular media to be between 10,000 and 50,000 people. U.S. FDA issues guidelines for maximum content.

2007: Pet food contamination triggers intensive research into chemically or physically determining presence of harmful agents & their metabolic pathways.

Mid 2000s: Metabolism of melamine and/or related compounds in bacteria metabolism studied in further detail using genetic analysis.

Mid 1990s: biotech interest in melamine metabolism due to possibility of enhancing plant nitrogen takeup in optimizing fertilization. Not much known about metabolic pathway. Nothing known about related gene expression. Bacterial strains known; Pseudomonas isolates, two Klebsiella isolates, and a Rhodococcus isolate).

Early 1990s: Melamine catabolism studied as means to degrade atrazine herbicide in the U.S. Pseudomonas used and first plasmids created.

Mid 1980s: HPLC analysis allows quantitative measurement of melamine pathway; prior to this, identification of intermediates is difficult.

Late 1970s: Partial, tentative, data into melamine degradation is published.


Design Team Schedule

  • Oct 08: Initial requirements & research
  • Nov 08: Set design goals. Initial dry lab design ideas.
  • Dec 08: Wet lab eval and initial measurements.
  • Jan 08 (planned): Fix dry lab design. Wet lab implementation.
  • Feb 08 (planned): Wet lab measurements from design. Feedback into model.
  • Mar 08 (planned): Report on working results.

Design Team Meetings

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