User:Jonathan Cline/Notebook/Ideal Microbe: Difference between revisions
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<td><td>ADP: Yes<br>Acido: ?<br>Casei: ? | <td><td>ADP: Yes<br>Acido: ?<br>Casei: ? | ||
<tr> | <tr> | ||
<tr> | |||
<td>Low hazard biosafety | |||
<td>Host can be purchased by individuals through procedures | |||
<td>MUST | |||
<td>Shelf life at least 1 year at 4C | <td>Shelf life at least 1 year at 4C | ||
<td>Allows easy archival and low cost lab setup | <td>Allows easy archival and low cost lab setup | ||
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<td> | <td> | ||
<td>(?) | <td>(?) | ||
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<td>Host can be readily supplied today | |||
<td>Obtaining the host is possible from ready stocks | |||
<td>hopefully | |||
</table> | </table> | ||
Revision as of 21:01, 9 November 2008
Please edit this page to add ideas / thoughts / corrections
- Working in a garage-lab or skunkworks environment imposes some restrictions on choice of organisms to engineer: expensive equipment/chemicals may be unavailable, protocols may need to be simplified to reduce man-hours, etc.
- There are many choices of host organisms for engineering, each having different strengths and weaknesses.
- Host organisms can be genetically improved for fitness, if a concise set of requirements are made, for engineering the organism.
- A great example is the "eau d'e coli" project from iGEM which removed (and subsequently enhanced!) the non-desirable smelly feature of the common BioBrick host.
Requirements for The Ideal Microbe
- "Must" means that the requirement must be met for proper acceptance.
- "Should" means that the requirement should attempt to be met, however operation is not jeopardized by failure to meet the requirement.
- "Hopefully" means that the requirement isn't necessary for operation, however is nice to have.
Feature | Details | Requirement | Candidates | ||
---|---|---|---|---|---|
Genome is 100% complete | Complete mapping means fewer unknowns, less trial-and-error, improved accuracy | MUST | ADP: Yes Acido: Yes Casei: Yes | ||
Pathway description is over 50% complete | Hard to quantify; though, "a lot is known" about the internals of the host. | MUST | |||
Non-pathogenic | Cloning and growth is not harmful to people. | MUST | ADP: Yes Acido: Yes Casei: Yes | ||
Transformable | Accepts vectors easily, makes engineering more precise. | MUST | |||
12 hr per dish growth rate | At ideal temperature, complete dish growth of wild type host. | MUST | |||
BioBrick Compatibility | Existing BB parts and protocols can be used | MUST | ADP: Yes Acido: ? Casei: ? | ||
Low hazard biosafety | Host can be purchased by individuals through procedures | MUST | Shelf life at least 1 year at 4C | Allows easy archival and low cost lab setup | MUST |
Low mutation rate | Lower mutation rate makes engineering more precise. | Should | |||
Room Temperature reproduction | Allows low cost lab setup | Should | |||
Electroporation | Allows modification using electroporation for low cost engineering | hopefully | |||
Cells can be freeze-dried | Allows simple shipment method and low cost lab setup | hopefully | |||
Public perceives host as "Friendly" | Hearing the name makes people smile rather than frown. | Should | ADP: No Acido: Yes Casei: Yes | ||
Doesn't smell bad | Indole production is limited or absent. | hopefully | ADP: No Acido: Maybe Casei: Maybe | ||
Looks translucent or opaque (or green?) | Doesn't invoke "yuck" when seen. | hopefully | |||
Gram-((negative or positive??)) | higher metabolic compatibility with existing parts, ??other?? | Should | ADP: Neg Acido: Pos Casei: Pos | ||
((Aerobic -or- Anerobic preference??)) | (?) | ||||
Host can be readily supplied today | Obtaining the host is possible from ready stocks | hopefully |
Suggested Microbes so far
- Acinetobacter sp. ADP1
- Lactobacillus acidophilus
- Lactobacillus casei
References
[Full Text] Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering. Nucleic Acids Research 2004 32(19):5780-5790; doi:10.1093/nar/gkh881