User:John Szymanski/notebook: Difference between revisions
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I made a culture for E.coli strain PRS305<br><br> | I made a culture for E.coli strain PRS305<br><br> | ||
==Registry of Standard Biological Parts== | |||
* http://parts.mit.edu | |||
When using biobrick parts, record the following: | |||
*Part # | |||
*Name of Part | |||
*Subparts | |||
*Location of Physical DNA: well, plate, and plasmid | |||
===Parts Used Today=== | |||
Group 3 | |||
a)BBa_J04630 | |||
IPTG Inducible promoter with RBS<br> | |||
LacI R0010 -> B0034<br> | |||
Plate 1, Well 16F, pSBIAK3<br> | |||
Revision as of 14:52, 6 February 2008
Lab Notebook
Making Plates
Label plates with date, antibiotic present. Lay out all plates with lids on top. Lift lid, pour ~3mm of agar/LB/antibiotic solution, and immediately put lid back on.
Cell Cultures
- LB solution with ampicillin
- sterile tubes with loose caps for air
- Label 2 tubes: Control, Strain of bacteria
- Sterilize pipette
- Pipette 3-10 mL of broth in each tube
- Mark colonies to be cultured on plate
- Dip scraper in ethanol and flame briefly, then let cool
- Crack the lid of the plate, scrape off the colony, then immediately inoculate culture
- incubate @ 37 C overnight with agitation
I made a culture for E.coli strain PRS305
Registry of Standard Biological Parts
When using biobrick parts, record the following:
- Part #
- Name of Part
- Subparts
- Location of Physical DNA: well, plate, and plasmid
Parts Used Today
Group 3
a)BBa_J04630
IPTG Inducible promoter with RBS
LacI R0010 -> B0034
Plate 1, Well 16F, pSBIAK3
