User:Jhdavis: Difference between revisions
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#[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | #[[Sauer:In_vitro_degradation_of_GFP-ssrA_by_ClpXP|ATP Regeneration Mix]] | ||
#[[Sauer:Making_yeg_plates|YEG-Cl]] | #[[Sauer:Making_yeg_plates|YEG-Cl]] | ||
#[[Sauer:Davis Minimal Media]] | |||
==Info== | ==Info== | ||
Revision as of 13:49, 1 May 2009
I recently joined the Sauer Lab. clpX holla!!!!
Protocols
- Western Blots by Eyal Jhdavis::Western::Eyal
- GFP Purification by organic extraction Jhdavis::GFPOrganic
- Expression/Prep of ClpXdN-linked Trimers Jhdavis::ClpXPrep
- Peptide cleavage Jhdavis::PeptideCleavage
- Flp recombinase Jhdavis::Flping
- ClpP his prep from Kim 2000 Jhdavis::ClpPHisPrep
- Sumo Protease expression/purification from Malakhov 2004 Jhdavis::SumoPrep
- regenerating qiagen columns Jhdavis::regenQiagen
- atpase notes Jhdavis::atpaseNotes
- Immunoprecipitation from eyal (sherman et. al. 1992 EMBO J) Jhdavis::Immunoprecip
Buffers
Info
- Jhdavis::GelFiltrationStandards
- Jhdavis::MetCleavage
- Jhdavis::lacIoperators
- Jhdavis::DNA 1 letter codes
- Jhdavis::Useful pymol
- Jhdavis::A260:A280 DNA:Protein ratios
- Jhdavis::fplc singup
