User:Jessica Karen Wong/Design

Constructing an output measurement kit derived from <BBpart> T9002</BBpart> consisting of

• an inducible promoter <BBpart> F2620</BBpart> which produces POPs in the presence of AHL
• a Biobrick site
• The ribosome binding site <BBpart> B0032</BBpart>
• The Green Fluorescent Protein <BBpart> E0040</BBpart>
• The terminator <BBpart> B0015</BBpart>

Assisting in the construction of

• Promoter tester derived from <BBpart> E0240</BBpart>
• RBS tester derived from <BBpart> I2055</BBpart>

Building devices containing RFP <BBpart> E1011</BBpart> and LacZ <BBpart> I0054</BBpart>

• Similar to <BBpart> E0240</BBpart> containing <BBpart> B0032</BBpart> and <BBpart> J04650</BBpart>
• Similar to <BBpart> I2055</BBpart> containing <BBpart> R0040</BBpart> and <BBpart> J04650</BBpart>

Progress

T90002

F2620________E X P1010 S P________E0040

Should be on both 3K3 and 1AK3

E0240

• Version 1 (E/S)
  • ___E CCDB S___RBS GFP T
  • Cut promoter E/S and insert
  • Can take part back out if necessary

• Version 2
  • ___E CCDB X___E0240
  • Cut Promoter E/S to insert
  • Forms mixed site so can't remove, real conditions

• Both versions should be on both 3K3 and 1AK3
• I2057 is the exact same but w/ RFP (both versions)

I2055

• Versions 1 (E/X)
  • __R0040___E CCDB X____GFP T
  • To insert RBS cut E/S, forms a mixed Spe/Xba site
  • Has too big spacing (full BB prefix) b/t promoter and RBS
  • Easier for testers to use

• Version 2 (S/X)
  • __R0040___S CCDB X____GFP T
  • Will get 2 spe/xba mixed sites, can't get RBS out
  • Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
  • Real condition spacing

• Both versions should be on both 3K3 and 1AK3
• Theoretically I2056 should be the same w/ RFP

Diagram