User:Jessica Karen Wong/Design: Difference between revisions
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(→T90002) |
(→E0240) |
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Line 25: | Line 25: | ||
==E0240== | ==E0240== | ||
*Version 1 (E/S) | *Version 1 (E/S) | ||
** | **___E CCDB S___RBS GFP T | ||
**Cut promoter E/S and insert | |||
**Can take part back out if necessary | |||
*Version 2 | |||
**___E CCDB X___E0240 | |||
**Cut Promoter E/S to insert | |||
**Forms mixed site so can't remove, real conditions |
Revision as of 12:32, 25 July 2007
Constructing an output measurement kit derived from <BBpart> T9002</BBpart> consisting of
- an inducible promoter <BBpart> F2620</BBpart> which produces POPs in the presence of AHL
- a Biobrick site
- The ribosome binding site <BBpart> B0032</BBpart>
- The Green Fluorescent Protein <BBpart> E0040</BBpart>
- The terminator <BBpart> B0015</BBpart>
Assisting in the construction of
- Promoter tester derived from <BBpart> E0240</BBpart>
- RBS tester derived from <BBpart> I2055</BBpart>
Building devices containing RFP <BBpart> E1011</BBpart> and LacZ <BBpart> I0054</BBpart>
- Similar to <BBpart> E0240</BBpart> containing <BBpart> B0032</BBpart> and <BBpart> J04650</BBpart>
- Similar to <BBpart> I2055</BBpart> containing <BBpart> R0040</BBpart> and <BBpart> J04650</BBpart>
T90002
F2620________E X P1010 S P________E0040
Should be on both 3K3 and 1AK3
E0240
- Version 1 (E/S)
- ___E CCDB S___RBS GFP T
- Cut promoter E/S and insert
- Can take part back out if necessary
- Version 2
- ___E CCDB X___E0240
- Cut Promoter E/S to insert
- Forms mixed site so can't remove, real conditions