User:Jessho: Difference between revisions

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Jessica Ho
===Basics===
Last name: Ho<br>
First name: Jessica<br>
Course/Minor: 20 and 8B.  No minor, yet.<br>
Email:jessho AT mit DOT edu<br>


===Last Name===
Have you taken or are you taking...<br>
Ho
Completed 7.05/5.07 (Biochemistry)<br>
Enrolled 7.06 (Cell Biology)<br>
No 7.02 (General Biology Lab)<br>
No 5.310 (General Chemistry Lab)<br>


===First Name===
Do you have any experience culturing cells (mammalian, yeast or microbial)?  Yes
Jessica


===Preferred name===
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?  Yes
Jess


===Course/Minor===
Please briefly describe any previous laboratory experience<br>
20 and 8B.  No minor, yet.
Undergrad team member, MIT iGEM 2007 team<br>
 
UROP, June 08 - Present, Lauffenburger Lab
===Year of Graduation===
2010
 
===Telephone #===
(703) 623-2414


===Email===
===20.109 Mod 2 - SAGA-Related info===
jessho AT mit DOT edu
My Favorite Protein: YJR097W


===Have you taken or are you taking...===
<u>Primer Design</u>
Completed 7.05/5.07 (Biochemistry)<br>
Enrolled 7.06 (Cell Biology)<br>
No 7.02 (General Biology Lab)<br>
No 5.310 (General Chemistry Lab)<br>


Do you have any experience culturing cells (mammalian, yeast or microbial)?<br>    Yes
Purpose: To design a primer to amplify a TAP TRP1 fusion gene.  After PCR, the fusion gene will have additional elements, i.e. homologous regions of the S. cerivisiae gene YGR097W flanking the TAP TRP1 gene, to enable C-terminus modification of YGR097W via homologous recombination.


Do you have any experience in molecular biology (electrophoresis, PCR, etc)?<br>  Yes
Forward:
5’- AAGGTTAATTTTGACATCGAGGAAGAGCAAGAAGGACAA TCC ATG GAA AAG AGA AGA TG - 3'


===Please briefly describe any previous laboratory experience===
Backward:
Undergrad team member, MIT iGEM 2007 team
5'- AGATTTATCTGATATGCTCAATTTCCCCTCCCATTTTCA GAC TCA CTA TAG GGC GA - 3'
UROP, June 08 - Present, Lauffenburger Lab


===Anything else you would like us to know?===
===20.109 Mod 3 Research Proposal===
I love experiential learning, so I'm super excited about 20.109!
Investigating the proteins with which extracellular Hsp90 interacts.
[[WFYellow(F08) Research Proposal | Click here for more info]]

Latest revision as of 14:25, 12 November 2008

Basics

Last name: Ho
First name: Jessica
Course/Minor: 20 and 8B. No minor, yet.
Email:jessho AT mit DOT edu

Have you taken or are you taking...
Completed 7.05/5.07 (Biochemistry)
Enrolled 7.06 (Cell Biology)
No 7.02 (General Biology Lab)
No 5.310 (General Chemistry Lab)

Do you have any experience culturing cells (mammalian, yeast or microbial)? Yes

Do you have any experience in molecular biology (electrophoresis, PCR, etc)? Yes

Please briefly describe any previous laboratory experience
Undergrad team member, MIT iGEM 2007 team
UROP, June 08 - Present, Lauffenburger Lab

20.109 Mod 2 - SAGA-Related info

My Favorite Protein: YJR097W

Primer Design

Purpose: To design a primer to amplify a TAP TRP1 fusion gene. After PCR, the fusion gene will have additional elements, i.e. homologous regions of the S. cerivisiae gene YGR097W flanking the TAP TRP1 gene, to enable C-terminus modification of YGR097W via homologous recombination.

Forward: 5’- AAGGTTAATTTTGACATCGAGGAAGAGCAAGAAGGACAA TCC ATG GAA AAG AGA AGA TG - 3'

Backward: 5'- AGATTTATCTGATATGCTCAATTTCCCCTCCCATTTTCA GAC TCA CTA TAG GGC GA - 3'

20.109 Mod 3 Research Proposal

Investigating the proteins with which extracellular Hsp90 interacts. Click here for more info

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