User:JeffreyLau: Difference between revisions

Revision as of 14:37, 14 June 2006

Undergraduate concentrating in Computer Science (class of 2007), member of 2006 Harvard iGem team.

Biobrick tutorial Note: more detailed procedure notes are in lab notebook.

Began with 12 samples (3x each of negative control, R0010, E0241, E7104). Fortunately our negative controls showed no growth overnight.

Made 9 minipreps for the remaining samples. Set aside the E7104 samples (they are a positive control).

Performed digests on 2x each of R0010 and E0241. We lost one of our E0241 samples by accident, so we threw out an R0010 sample to balance.

Added Calf Intestinal Phosphatase (CIP) to R0010 samples to prevent self-ligation of the R0010 vector.

Notes on using the nanodrop

Nanodrop measures concentration of DNA in small samples (1 µL). It is apparently not very accurate.
Clean nanodrop thoroughly with kimwipes and ethanol, before and after each use
On desktop, "ND-1000"->"Nucleic Acid"
Before each set of samples:
- Calibrate nanodrop with drop of distilled water
- Calibrate nanodrop with drop of background (solute), e.g. distilled water or elution buffer
Add sample to nanodrop, enter name of sample, "Measure"
- Note: mix sample before placing on nanodrop

@@ Line 3: / Line 3: @@
 == Lab notebook ==
-'''2006/06/14'''
+== 2006/06/14 ==
-<p>Began with 12 samples (3x each of negative control, R0010, E0241, E7104). Negative controls showed no growth overnight</p>
+'''Biobrick tutorial'''
+Note: more detailed procedure notes are in lab notebook.
+*Began with 12 samples (3x each of negative control, R0010, E0241, E7104). Fortunately our negative controls showed no growth overnight.
+*Made 9 minipreps for the remaining samples. Set aside the E7104 samples (they are a positive control).
+*Performed digests on 2x each of R0010 and E0241. We lost one of our E0241 samples by accident, so we threw out an R0010 sample to balance.
+*Added Calf Intestinal Phosphatase (CIP) to R0010 samples to prevent self-ligation of the R0010 vector.
+*Performed gel electrophoresis on R0010 and E0241 samples.
+'''Notes on using the nanodrop'''
+*Nanodrop measures concentration of DNA in small samples (1 µL). It is apparently not very accurate.
+*Clean nanodrop thoroughly with kimwipes and ethanol, before and after each use
+*On desktop, "ND-1000"->"Nucleic Acid"
+*Before each set of samples:
+**Calibrate nanodrop with drop of distilled water
+**Calibrate nanodrop with drop of background (solute), e.g. distilled water or elution buffer
+*Add sample to nanodrop, enter name of sample, "Measure"
+**Note: mix sample before placing on nanodrop