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Revision as of 10:12, 2 November 2006
Bio
Jason Kelly
4th Year Biological Engineering Graduate Student @ MIT in the Endy Lab
S.B. in Biology and Chemical Engineering @ MIT, 2003
Contact
jasonk AT mit DOT edu
Activities
OpenWetWare steering committee coordinator - please help out!
Thesis Project
To date, engineered biological systems have been constructed via a variety of ad hoc approaches. The resulting systems should be thought of as pieces of art. We are interested in exploring how existing forward engineering approaches might be best combined with directed evolution to make routine the construction of engineered biological systems. We have specified a procedure for construction of biological systems via screening of subcomponent libraries and rational re-assembly. We have begun development of tools to enable this approach, including a FACS-based screening system to rapidly measure the input/output function of a genetic circuit. Additionally, we have designed a microfluidic system that enables more sophisticated screening and selection functions. Specifically, a microfluidic chemostat integrated with a cell sorter (i.e., a sort-o-stat). This microscope-based system will enable us to evaluate whether or not more complicated screens and selections will be of practical use in service of evolving engineered biological systems.
Specific Aims
Communications
Current Project Pages
- Endy:Evolutionary stability project
- Sortostat/Growth Tests
- Knight:Evolving Reshmaverters
- Endy:Chemostat
- Endy:Screening plasmid 2.0
OWW Pages in Progess
BioBricks construction tutorial - a step-by-step tutorial for a single Biobrick assembly.
Microfluidics - a page describing the endy lab microfluidics setup and linking to relevant protocols, etc.
BE Board - homepage for the BE Board
OpenWetWare:Presentations - a presentation for introducing new labs to Openwetware.
ICampus Application for OWW - application for an MIT iCampus grant for OpenWetWare (and a good desciption of the site and future goals)
Science 2.0 - some musings by the OWW community about the internet (Web 2.0) and science
Community Portal v2 - playing around with Community portal organization.
BE Board:Dinner Discussion/Alternate publishing models - discussion for BE retreat
User:Jasonk/sidebar - my sidebar, this is a great feature!
OpenWetWare:Steering committee/NSF BDI Grant - help us write a grant for OWW.
OpenWetWare:Steering committee/Goals
OpenWetWare:Presentations/SciFoo - walkthough of the site shown at Sci Foo camp
Jason Kelly/sample wikipedia page for OWW - wikipedia entry for OWW
OpenWetWare:Steering Committee v2 - updating SC webpage
edit your sidebar - would be nice to make it easy for users to figure out how to edit their sidebar, though apparently this isn't the best solution.
Postgenomic test page - testing having our content scraped by postgenomic.
Glycerols
Papers of Interest
Library-based Construction
Evolutionary Stability
Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)
Random
MIT audio bridge conference calling
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