User:Jarle Pahr/Transformation: Difference between revisions
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http://openwetware.org/wiki/IGEM:Paris_Bettencourt_2012/Protocols/Heat_Shock_Transformation_of_E_Coli | http://openwetware.org/wiki/IGEM:Paris_Bettencourt_2012/Protocols/Heat_Shock_Transformation_of_E_Coli | ||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC145660/ | |||
http://2010.igem.org/wiki/images/a/ae/Direct_Plating_Transformation_Protocol.pdf |
Revision as of 11:21, 25 February 2013
Notes on bacterial transformation:
1 h incubation has been mentioned to be sufficient for recovery of Kan resistant transformants:
http://www.protocol-online.org/biology-forums/posts/39293.html
See also http://www.protocol-online.org/biology-forums/posts/24469.html
Kanamycin works by interfering with protein translation.
http://www.genome.ou.edu/protocol_book/protocol_adxF.html
http://www.protocol-online.org/biology-forums/posts/25521.html
http://www.ncbi.nlm.nih.gov/pubmed/12065899
http://bitesizebio.com/articles/plasmid-retention/
http://www.protocol-online.org/biology-forums/posts/24469.html
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC145660/
http://2010.igem.org/wiki/images/a/ae/Direct_Plating_Transformation_Protocol.pdf