User:Jarle Pahr/Transformation

Notes on bacterial transformation:

1 h incubation has been mentioned to be sufficient for recovery of Kan resistant transformants: http://www.protocol-online.org/biology-forums/posts/39293.html

See also http://www.protocol-online.org/biology-forums/posts/24469.html

Kanamycin works by interfering with protein translation.

http://www.genome.ou.edu/protocol_book/protocol_adxF.html

http://www.protocol-online.org/biology-forums/posts/25521.html

http://www.ncbi.nlm.nih.gov/pubmed/12065899

http://bitesizebio.com/articles/plasmid-retention/

http://www.protocol-online.org/biology-forums/posts/24469.html

http://openwetware.org/wiki/IGEM:Paris_Bettencourt_2012/Protocols/Heat_Shock_Transformation_of_E_Coli

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC145660/

http://2010.igem.org/wiki/images/a/ae/Direct_Plating_Transformation_Protocol.pdf

High efficiency transformation of Escherichia coli with plasmids: http://www.sciencedirect.com/science/article/pii/037811199090336P

Commercial competent cells

Name	Description/Use	Supplier	(Main) Genotypes	Transformation efficiency (cfu/ug)	Comment	Reference
XL10-GOLD Ultracompetent cells	Chemocompetent cells	Stratagene	Hte	>5x10^9		http://www.freewebs.com/labjuan2/XL10%20competent%20cells%20STRATAGENE.pdf

Bibliography

One-step preparation of competent Escherichia coli:Transformation and storage of bacteria lcells in the same solution: http://www.pnas.org/content/86/7/2172.full.pdf