User:Jarle Pahr/Strains: Difference between revisions
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|BW25083|| || || || | |BW25083|| || || || | ||
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|[http://ecoliwiki.net/colipedia/index.php/BW25113 BW25113]|| || ||[[http://ecoliwiki.net/colipedia/index.php/BW25113 BW25113 at Ecoliwiki] || | |[http://ecoliwiki.net/colipedia/index.php/BW25113 BW25113]|| || ||[[http://ecoliwiki.net/colipedia/index.php/BW25113 BW25113 at Ecoliwiki] ||Parent strain of the Keio single-deletion mutant collection. | ||
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|BW21578||rrnBT14 ΔlacZWJ16||BD792 via BW21342|| || | |BW21578||rrnBT14 ΔlacZWJ16||BD792 via BW21342|| || | ||
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|[http://cgsc.biology.yale.edu/Strain.php?ID=65553 BW26434] (CGSC #7658)||Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514|| || || | |[http://cgsc.biology.yale.edu/Strain.php?ID=65553 BW26434] (CGSC #7658)||Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514|| || || | ||
|- | |- | ||
|[http://cgsc.biology.yale.edu/Strain.php?ID=72167 BW27784]|| || || || | |[http://cgsc.biology.yale.edu/Strain.php?ID=72167 BW27784]|| || || ||Expresses arabinose transporter constitutively. | ||
|- | |- | ||
|[http://cgsc.biology.yale.edu/Strain.php?ID=72168 BW22786]|| || || || | |[http://cgsc.biology.yale.edu/Strain.php?ID=72168 BW22786]|| || || ||Expresses arabinose transporter constitutively. | ||
|} | |} | ||
Revision as of 01:48, 16 July 2013
Notes on various bacterial strains:
E. coli:
| Strain | Description | Heritage | References | Comments |
|---|---|---|---|---|
| Dh5a | ||||
| BW21342 | ||||
| BW25083 | ||||
| BW25113 | [BW25113 at Ecoliwiki | Parent strain of the Keio single-deletion mutant collection. | ||
| BW21578 | rrnBT14 ΔlacZWJ16 | BD792 via BW21342 | ||
| BW22773 | lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132; | |||
| BW26434 (CGSC #7658) | Δ(araD-araB)567, Δ(lacA-lacZ)514(::kan), lacIp-4000(lacIq), λ-, rpoS396(Am)?, rph-1, Δ(rhaD-rhaB)568, hsdR514 | |||
| BW27784 | Expresses arabinose transporter constitutively. | |||
| BW22786 | Expresses arabinose transporter constitutively. |
Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):
"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.
As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.
Nomenclature:
- Insertion mutation.
Bibliography:
Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long
http://web.mit.edu/7.02/pdfs/nomenclature.pdf
CGSC - Coli Genetic Stock Center: http://cgsc.biology.yale.edu/index.php
http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp
Verification
Mutation-specific primers for verification of strains by PCR:
