User:Jarle Pahr/Strains: Difference between revisions
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'''E. coli:''' | |||
BW27784 | {| class="wikitable" border="1" cellpadding="5" cellspacing="0" | ||
|- | |||
! Strain!! Description !! Heritage !! References | |||
|- | |||
|Dh5a|| || | |||
|- | |||
|- | |||
|BW21578||rrnBT14 ΔlacZWJ16||BD792 via BW21342|| | |||
|BW22773||lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132;|| || | |||
|BW27784|| || || | |||
|- | |||
|BW22786|| || || | |||
|} | |||
Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes): | |||
"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113. | |||
As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+. | |||
Revision as of 08:19, 31 May 2013
Notes on various bacterial strains:
E. coli:
| Strain | Description | Heritage | References | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Dh5a | |||||||||||
| BW21578 | rrnBT14 ΔlacZWJ16 | BD792 via BW21342 | BW22773 | lacIq rrnBT14 ΔlacZWJ16 proC::Tn5-132; | BW27784 | ||||||
| BW22786 |
Note on LacI satus of BW strains (copied from http://openwetware.org/wiki/E._coli_genotypes):
"We have now confirmed that BW25113, BW25141, and BW26434 are all lacI+, and not lacIq. We thank you for alerting us to the error with respect to BW26434. Apparently, the lacI region was restored to wild-type in a predecessor of BW25113.
As strains BW27784 and BW22786 were also constructed from BW25113, the former should also be LacI+.
Bibliography:
Khlebnikov 2001: http://mic.sgmjournals.org/content/147/12/3241.long
