User:Jarle Pahr/Restriction

Notes on procedures and issues relating to restriction digests:

http://en.wikipedia.org/wiki/Restriction_digest

About star activity: https://www.neb.com/tools-and-resources/usage-guidelines/star-activity

Double Digest tool: http://www.thermoscientificbio.com/webtools/doubledigest/

Enzyme volume should not exceed 10 % of total reaction volume, due to glycerol content.

NEB Double Digest finder: https://www.neb.com/tools-and-resources/interactive-tools/double-digest-finder

http://utminers.utep.edu/rwebb/html/restriction_digestion.html

http://www.molecularstation.com/dna/restriction-enzyme-digestion/

iGEM - restriction digest protocol: http://partsregistry.org/Help:Protocols/Restriction_Digest

Sequence Manipulation Suite: Restriction Digest: http://www.bioinformatics.org/sms2/rest_digest.html

http://molbiol-tools.ca/Restriction_endonuclease.htm

http://www.promega.com/resources/product-guides-and-selectors/restriction-enzyme-resource/restriction-enzyme-general-information/

Supercoiled plasmid DNA migrates faster than linear (ex cut plasmid) DNA: http://www.researchgate.net/post/Does_supercoiled_dna_migrate_faster_in_agarose_gel_electrophoresis_than_linear_form_of_dna

https://www.neb.com/tools-and-resources/usage-guidelines/cleavage-close-to-the-end-of-dna-fragments

Enzyme	Recognition site	Buffers
AgeI	ACCGGT	Example
PciI	ACATGT	Example
EcoRI		Example