User:Jarle Pahr/Recipes: Difference between revisions
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LB (1000 mL): | LB (1000 mL): | ||
10 g tryptone | 10 g tryptone | ||
5 g yeast extract | 5 g yeast extract | ||
5 g NaCl | 5 g NaCl | ||
Terrfic Broth (http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620.full?text_only=true): | |||
900 mL H2O | |||
12 g tryptone | |||
24 g yeast extract | |||
4 mL glycerol | |||
Autoclave and add 100 mL sterile solution 0.17 M KH2PO4, 0.72 M K2HPO4. | |||
0.17 :M KH2PO4, 0.72 M K2HPO4: | |||
2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. Dissolve salts, adjust volume to 100 mL and autoclave. | |||
Latest revision as of 06:48, 9 May 2013
LB (1000 mL): 10 g tryptone 5 g yeast extract 5 g NaCl
Terrfic Broth (http://cshprotocols.cshlp.org/content/2006/1/pdb.rec8620.full?text_only=true):
900 mL H2O 12 g tryptone 24 g yeast extract 4 mL glycerol
Autoclave and add 100 mL sterile solution 0.17 M KH2PO4, 0.72 M K2HPO4.
0.17 :M KH2PO4, 0.72 M K2HPO4:
2.31 g of KH2PO4 and 12.54 g of K2HPO4 in 90 mL of H2O. Dissolve salts, adjust volume to 100 mL and autoclave.
