User:Jarle Pahr/RF: Difference between revisions
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Notes on Restriction Free (RF) Cloning: | |||
For primers designed by rf-cloning.org, the annealing temperature for the primary stage will generally be ~ 55 Cm, and ~ 60 C for the secondary stage. | |||
Latest revision as of 09:46, 19 February 2013
Notes on Restriction Free (RF) Cloning:
For primers designed by rf-cloning.org, the annealing temperature for the primary stage will generally be ~ 55 Cm, and ~ 60 C for the secondary stage.
