User:Jarle Pahr/Questions: Difference between revisions
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Given a certain mutation rate, for how long can one expect maintain 100 % sequence preservation in a plasmid. | |||
When working with plasmids, a very large number of DNA molecules is handled simultaneously, but mutations occur in discrete molecules. What are the "population dynamics" of mutations? | |||
Revision as of 07:55, 20 March 2013
Technical and scientific questions which can conceivable by answered by in silico or small-scale, low-cost wetlab experiments:
Non-standard growth media: Can E. coli grow on "dextro" energy tablets?
Verification of commercial personal-genomics results: Can DNA extraction, PCR and sequencing confirm a SNP result reported by 23andme?
Commercial kit performance: What are the performance properties of various commercial molecular-biology kits?
Can horse meat be reliably detected by a cheap, at-home PCR test?
Do dNTPs show up during gel electrophoresis?
pllD: Can activity of the pllD promoter be shown by expression of GFP in a minimal medium?
Given a certain mutation rate, for how long can one expect maintain 100 % sequence preservation in a plasmid.
When working with plasmids, a very large number of DNA molecules is handled simultaneously, but mutations occur in discrete molecules. What are the "population dynamics" of mutations?
What organisms live in a home environment? Can culturing, PCR and 16S sequencing be used to identify those organisms?
Big questions
Oligonucleotide synthesis: Will there be further radical improvement?
