User:Jarle Pahr/Promoters

Notes on various promoters:

OmpA: Among the strongest promoters in E. coli (1, cited in 2).

Reporter systems

http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html

(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html )

pBAD

• Inducible by l-arabinose
• Wild-type E. coli show heterogenous (all-or-nothing) induction upon addition of arabinose.
• Strains for homogenous induction by arabinose have been developed.
• Subject to catabolite repression.

Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94830/

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.: http://jb.asm.org/content/177/14/4121.full

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345380/

Repression and catabolite gene activation in the araBAD operon. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211852/

Promoter sequences

Description	Length (bp)	Sequence	Location (absolute)	Location (relative)	Comment
LacUV5					Constitutive promoter
rrnB P1 73 bp fragment	gttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccact			-70 to +3	Strong promoter. Inhibited by ppGpp.
GreA promoter 60bp fragment	ggcgcaacgccctataaagtaaacgatgacccttcgggaacttcagggtaaaatgactAt			-58 to +2
PcnB
MazEF
IraP
His
Thr

Links/bibliography

http://wolfson.huji.ac.il/expression/vector/promoters.html