User:Jarle Pahr/Promoters: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jarle Pahr (talk | contribs) (→pBAD) |
Jarle Pahr (talk | contribs) |
||
| (One intermediate revision by the same user not shown) | |||
| Line 2: | Line 2: | ||
OmpA: Among the strongest promoters in E. coli ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341223/pdf/nar00303-0094.pdf 1], cited in [http://www.biomedcentral.com/1472-6750/13/12 2]). | OmpA: Among the strongest promoters in E. coli ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341223/pdf/nar00303-0094.pdf 1], cited in [http://www.biomedcentral.com/1472-6750/13/12 2]). | ||
=Reporter systems= | |||
http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html | |||
(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html ) | |||
| Line 26: | Line 33: | ||
==Lac promoter== | ==Lac promoter== | ||
=lldP= | =lldP= | ||
Latest revision as of 11:39, 15 July 2013
Notes on various promoters:
OmpA: Among the strongest promoters in E. coli (1, cited in 2).
Reporter systems
http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html
(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html )
XylS/Pm system
pBAD
- Inducible by l-arabinose
- Wild-type E. coli show heterogenous (all-or-nothing) induction upon addition of arabinose.
- Strains for homogenous induction by arabinose have been developed.
- Subject to catabolite repression.
Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94830/
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.: http://jb.asm.org/content/177/14/4121.full
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345380/
Repression and catabolite gene activation in the araBAD operon. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211852/
Lac promoter
lldP
GreA
Promoter sequences
| Description | Length (bp) | Sequence | Location (absolute) | Location (relative) | Comment |
|---|---|---|---|---|---|
| LacUV5 | Constitutive promoter | ||||
| rrnB P1 73 bp fragment | gttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccact | -70 to +3 | Strong promoter. Inhibited by ppGpp. | ||
| GreA promoter 60bp fragment | ggcgcaacgccctataaagtaaacgatgacccttcgggaacttcagggtaaaatgactAt | -58 to +2 | |||
| PcnB | |||||
| MazEF | |||||
| IraP | |||||
| His | |||||
| Thr |
