User:Jarle Pahr/Promoters: Difference between revisions
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Jarle Pahr (talk | contribs) (New page: Notes on various promoters: OmpA: Among the strongest promoters in E. coli ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341223/pdf/nar00303-0094.pdf 1], cited in [http://www.biomedcentr...) |
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Notes on various promoters: | Notes on various promoters: | ||
OmpA: Among the strongest promoters in E. coli ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341223/pdf/nar00303-0094.pdf 1], cited in [http://www.biomedcentral.com/1472-6750/13/12 2]). | OmpA: Among the strongest promoters in E. coli ([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341223/pdf/nar00303-0094.pdf 1], cited in [http://www.biomedcentral.com/1472-6750/13/12 2]). | ||
=Reporter systems= | |||
http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html | |||
(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html ) | |||
==XylS/Pm system== | |||
==pBAD== | |||
*Inducible by l-arabinose | |||
*Wild-type E. coli show heterogenous (all-or-nothing) induction upon addition of arabinose. | |||
*Strains for homogenous induction by arabinose have been developed. | |||
*Subject to catabolite repression. | |||
Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94830/ | |||
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.: http://jb.asm.org/content/177/14/4121.full | |||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345380/ | |||
Repression and catabolite gene activation in the araBAD operon. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211852/ | |||
==Lac promoter== | |||
=lldP= | |||
=GreA= | |||
=Promoter sequences= | |||
{| class="wikitable" border="1" cellpadding="5" cellspacing="0" | |||
|- | |||
! Description !! Length (bp) !! Sequence !! Location (absolute)!! Location (relative)!! Comment | |||
|- | |||
|LacUV5|| || || || ||Constitutive promoter | |||
|- | |||
|rrnB P1 73 bp fragment||gttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccact|| || ||-70 to +3 ||Strong promoter. Inhibited by ppGpp. | |||
|- | |||
|GreA promoter 60bp fragment||ggcgcaacgccctataaagtaaacgatgacccttcgggaacttcagggtaaaatgactAt|| || ||-58 to +2 || | |||
|- | |||
|PcnB|| || || || || | |||
|- | |||
|MazEF|| || || || || | |||
|- | |||
|IraP|| || || || || | |||
|- | |||
|His|| || || || || | |||
|- | |||
|Thr|| || || || || | |||
|} | |||
=Links/bibliography= | |||
http://wolfson.huji.ac.il/expression/vector/promoters.html | |||
Latest revision as of 11:39, 15 July 2013
Notes on various promoters:
OmpA: Among the strongest promoters in E. coli (1, cited in 2).
Reporter systems
http://www.nature.com/nmeth/journal/v3/n8/full/nmeth895.html
(data downloadable at http://www.weizmann.ac.il/mcb/UriAlon/index.html )
XylS/Pm system
pBAD
- Inducible by l-arabinose
- Wild-type E. coli show heterogenous (all-or-nothing) induction upon addition of arabinose.
- Strains for homogenous induction by arabinose have been developed.
- Subject to catabolite repression.
Regulatable Arabinose-Inducible Gene Expression System with Consistent Control in All Cells of a Culture: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC94830/
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.: http://jb.asm.org/content/177/14/4121.full
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC345380/
Repression and catabolite gene activation in the araBAD operon. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211852/
Lac promoter
lldP
GreA
Promoter sequences
| Description | Length (bp) | Sequence | Location (absolute) | Location (relative) | Comment |
|---|---|---|---|---|---|
| LacUV5 | Constitutive promoter | ||||
| rrnB P1 73 bp fragment | gttgcgcggtcagaaaattattttaaatttcctcttgtcaggccggaataactccctataatgcgccaccact | -70 to +3 | Strong promoter. Inhibited by ppGpp. | ||
| GreA promoter 60bp fragment | ggcgcaacgccctataaagtaaacgatgacccttcgggaacttcagggtaaaatgactAt | -58 to +2 | |||
| PcnB | |||||
| MazEF | |||||
| IraP | |||||
| His | |||||
| Thr |
