User:Jarle Pahr/Plasmids

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Plasmid Description Length (bp) Origin of Replication Selection markers Comment
pJP-1 Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI oriV (RK2) KanR
pJP-2 Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR
pJP-3 Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6093 oriV (RK2) KanR
pJP-4 Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6091 oriV (RK2) KanR
pJP-5 Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-6 Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-7 Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-8 Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6118 oriV (RK2) KanR
pJP-9 Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR
pJP-10 Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI 6095 oriV (RK2) KanR