User:Jarle Pahr/Plasmids

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|pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| ||
|pJP-11||Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI||7867||oriV (R2)||KanR|| ||
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pSB-M1g

Revision as of 10:29, 21 May 2013

Plasmid Description Length (bp) Origin of Replication Selection markers Data Comment
pJP-1Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI oriV (RK2) KanR6IE58_.fas
pJP-2Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR76IE57_.fas
pJP-3Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6093 oriV (RK2) KanR
pJP-4Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6091 oriV (RK2) KanR
pJP-5Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-6Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-7Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6106 oriV (RK2) KanR
pJP-8Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6118 oriV (RK2) KanR
pJP-9Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI 6105 oriV (RK2) KanR
pJP-10Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI 6095 oriV (RK2) KanR
pJP-11Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI7867oriV (R2)KanR



pSB-M1g

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