User:Jarle Pahr/Plasmids: Difference between revisions
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=Bibliography= | =Bibliography= | ||
http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf | Identification and Classification of Bacterial Plasmids: http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf | ||
Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/ | Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/ |
Revision as of 12:08, 3 June 2013
Plasmid | Description | Length (bp) | Origin of Replication | Selection markers | Data | Comment |
---|---|---|---|---|---|---|
pJP-1 | Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI | oriV (RK2) | KanR | 6IE58_.fas | ||
pJP-2 | Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | 76IE57_.fas | |
pJP-3 | Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6093 | oriV (RK2) | KanR | ||
pJP-4 | Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6091 | oriV (RK2) | KanR | ||
pJP-5 | Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
pJP-6 | Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
pJP-7 | Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | ||
pJP-8 | Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6118 | oriV (RK2) | KanR | ||
pJP-9 | Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | ||
pJP-10 | Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI | 6095 | oriV (RK2) | KanR | ||
pJP-11 | Ligation of GFP-LVA PCR product cut KpnI BamHI into pSB-M1g cut KpnI BamHI | 7867 | oriV (R2) | KanR |
pSB-M1g
pUC19:
pUC118:
pUC128: http://www.ncbi.nlm.nih.gov/pubmed/2853689
pUM9
pUM66
Bibliography
Identification and Classification of Bacterial Plasmids: http://pubmedcentralcanada.ca/pmcc/articles/PMC373151/pdf/microrev00046-0077.pdf
Microbiol Mol Biol Rev. 1998 June; 62(2): 434–464. PMCID: PMC98921. Replication and Control of Circular Bacterial Plasmids: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/