User:Jarle Pahr/Plasmids: Difference between revisions
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Jarle Pahr (talk | contribs) (New page: {| class="wikitable" border="1" cellpadding="5" cellspacing="0" |- ! Plasmid!! Description !!Length (bp) !! Origin of Replication !! Selection markers!! Comment |- |pJP-1|| || ||oriV...) |
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! Plasmid!! Description !!Length (bp) !! Origin of Replication !! Selection markers!! Comment | ! Plasmid!! Description !!Length (bp) !! Origin of Replication !! Selection markers!! Comment | ||
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|pJP-1|| || ||oriV (RK2) ||KanR|| | |pJP-1||Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI || ||oriV (RK2) ||KanR|| | ||
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|pJP-2|| || ||oriV (RK2) ||KanR|| | |pJP-2||Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| | ||
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|pJP-3|| || ||oriV (RK2) ||KanR|| | |pJP-3||Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6093 ||oriV (RK2) ||KanR|| | ||
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|pJP-4|| || ||oriV (RK2) ||KanR|| | |pJP-4||Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6091 ||oriV (RK2) ||KanR|| | ||
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|pJP-5|| || ||oriV (RK2) ||KanR|| | |pJP-5||Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | ||
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|pJP-6|| || ||oriV (RK2) ||KanR|| | |pJP-6||Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | ||
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|pJP-7|| || ||oriV (RK2) ||KanR|| | |pJP-7||Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6106 ||oriV (RK2) ||KanR|| | ||
|- | |- | ||
|pJP-8|| || ||oriV (RK2) ||KanR|| | |pJP-8||Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6118 ||oriV (RK2) ||KanR|| | ||
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|pJP-9|| || ||oriV (RK2) ||KanR|| | |pJP-9||Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI ||6105 ||oriV (RK2) ||KanR|| | ||
|- | |- | ||
|pJP-10|| || ||oriV (RK2) ||KanR|| | |pJP-10||Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI ||6095 ||oriV (RK2) ||KanR|| | ||
|} | |} | ||
Revision as of 09:12, 2 May 2013
| Plasmid | Description | Length (bp) | Origin of Replication | Selection markers | Comment |
|---|---|---|---|---|---|
| pJP-1 | Ligation of rrnB P1 74 bp fragment into pSB-M1g cut AgeI PciI | oriV (RK2) | KanR | ||
| pJP-2 | Ligation of GreA 60 bp fragment cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | |
| pJP-3 | Ligation of lacUV5 PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6093 | oriV (RK2) | KanR | |
| pJP-4 | Ligation of "ArgI 46 bp fragment" PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6091 | oriV (RK2) | KanR | |
| pJP-5 | Ligation of IraP PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | |
| pJP-6 | Ligation of livJ 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | |
| pJP-7 | Ligation of His 61 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6106 | oriV (RK2) | KanR | |
| pJP-8 | Ligation of Thr 73 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6118 | oriV (RK2) | KanR | |
| pJP-9 | Ligation of MazEF 60 bp fragment PCR product cut AgeI PciI into pSB-M1g cut AgeI PciI | 6105 | oriV (RK2) | KanR | |
| pJP-10 | Ligation of pcnB 50bp fragment PCR product cut AgeI pciI into pSB-M1g cut AgeI PciI | 6095 | oriV (RK2) | KanR |
