User:Jarle Pahr/Microbiology
Notes on microbiology:
http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0059310
Rapid microbiological methods: http://www.rapidmicrobiology.com/test-methods/Rapid-Method-Pharma.php
When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant Escapes Detection Even with Deep Genome Sequencing: http://mbio.asm.org/content/4/2/e00591-12.abstract
http://cellculture.bitesizebio.com/articles/cell-culture-passage-number-explained/
Concepts/Glossary
- Passage number
Strain collections
American Type Culture Collection (ATCC): http://www.lgcstandards-atcc.org/Products/Cells%20and%20Microorganisms/Primary%20Cells.aspx?geo_country=no
NCMA (Previously CCMP): https://ncma.bigelow.org/
Microbiology-themed blogs: http://www.microbe.net/microbiology-blogs/
Growth data
Growth data from cultures:
Strain/plasmid | Date | Volume | Medium | Container | Incubation conditions | Incubation time | OD600 | # of samples used for measuring OD600 | Comment |
---|---|---|---|---|---|---|---|---|---|
Dh5a:pJP-3 | 22.5.13 | 5 mL | LB + 50 ug/mL Kan | 13 mL tube | 37 C, 225 RPM | ~ 13 h | 1 | ||
BW27784 | 1.6.13 | 5 mL | LB | 13 mL tube | 37 C, 225 RPM | ON | 7,7 | ||
BW27786 | 1.6.13 | 5 mL | LB | 13 mL tube | 37 C, 225 RPM | ON | 2,9 | ||
BW27784 | 08.6.13 | ~3 mL | LB | 13 mL tube | ~20 C (on bench) | ON | 0,9 | 1 |
Educational
http://invisiblelife.yourwildlife.org/
http://media.hhmi.org/biointeractive/vlabs/bacterial_id/index.html
Handbooks and references
http://kunde.datakvalitet.no/SIHF/Mikrobiologi/
Blogs
http://covertlab.stanford.edu/publicationpdfs/ChaosJKarr2013.pdf
microBEnet blog:
http://www.microbe.net/microbenet-blog/
Links
http://www.earthmicrobiome.org/
http://www.microbemagazine.org/
American society for Microbiology (ASM): www.asm.org
http://schaechter.asmblog.org/schaechter/2010/07/the-uncultured-bacteria.html
Bibliography
Draft Genome Sequence of an Actinobacterium, Brachybacterium muris Strain UCD-AY4 : http://genomea.asm.org/content/1/2/e00086-13.full