User:Jarle Pahr/Microbiology

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Notes on microbiology:


http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0059310


Rapid microbiological methods: http://www.rapidmicrobiology.com/test-methods/Rapid-Method-Pharma.php

When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant Escapes Detection Even with Deep Genome Sequencing: http://mbio.asm.org/content/4/2/e00591-12.abstract


http://cellculture.bitesizebio.com/articles/cell-culture-passage-number-explained/


Concepts/Glossary

  • Passage number


Strain collections

American Type Culture Collection (ATCC): http://www.lgcstandards-atcc.org/Products/Cells%20and%20Microorganisms/Primary%20Cells.aspx?geo_country=no

NCMA (Previously CCMP): https://ncma.bigelow.org/


Microbiology-themed blogs: http://www.microbe.net/microbiology-blogs/



Growth data

Growth data from cultures:


Strain/plasmid Date Volume Medium Container Incubation conditions Incubation time OD600 # of samples used for measuring OD600 Comment
Dh5a:pJP-3 22.5.13 5 mL LB + 50 ug/mL Kan 13 mL tube 37 C, 225 RPM ~ 13 h 1
BW27784 1.6.13 5 mL LB 13 mL tube 37 C, 225 RPM ON 7,7
BW27786 1.6.13 5 mL LB 13 mL tube 37 C, 225 RPM ON 2,9

Educational

http://media.hhmi.org/biointeractive/vlabs/bacterial_id/index.html

Handbooks and references

http://kunde.datakvalitet.no/SIHF/Mikrobiologi/

Blogs

http://microbepost.org