User:Jarle Pahr/Microbiology: Difference between revisions
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{| class="wikitable" border="1" cellpadding="5" cellspacing="0" | {| class="wikitable" border="1" cellpadding="5" cellspacing="0" | ||
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! | ! Strain/plasmid!! Date !! Volume !! Medium !! Container !! Incubation conditions !! Incubation time !! OD600 !! # of samples used for measuring OD600!! Comment | ||
|- | |- | ||
| Dh5a:pJP-3 || 22.5.13 || 5 mL || LB + 50 ug/mL Kan || 13 mL tube ||37 C, 225 RPM ||~ 13 h || ||1 || | | Dh5a:pJP-3 || 22.5.13 || 5 mL || LB + 50 ug/mL Kan || 13 mL tube ||37 C, 225 RPM ||~ 13 h || ||1 || | ||
|- | |||
|BW27784||1.6.13||5 mL|| LB||13 mL tube||37 C, 225 RPM|| ON||7,7|| | |||
|- | |||
||BW27786||1.6.13||5 mL|| LB||13 mL tube||37 C, 225 RPM|| ON||2,9|| | |||
|} | |} | ||
=Educational= | =Educational= |
Revision as of 06:38, 2 June 2013
Notes on microbiology:
http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0059310
When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant Escapes Detection Even with Deep Genome Sequencing: http://mbio.asm.org/content/4/2/e00591-12.abstract
http://cellculture.bitesizebio.com/articles/cell-culture-passage-number-explained/
Concepts/Glossary
- Passage number
Strain collections
American Type Culture Collection (ATCC): http://www.lgcstandards-atcc.org/Products/Cells%20and%20Microorganisms/Primary%20Cells.aspx?geo_country=no
NCMA (Previously CCMP): https://ncma.bigelow.org/
Microbiology-themed blogs: http://www.microbe.net/microbiology-blogs/
Growth data
Growth data from cultures:
Strain/plasmid | Date | Volume | Medium | Container | Incubation conditions | Incubation time | OD600 | # of samples used for measuring OD600 | Comment |
---|---|---|---|---|---|---|---|---|---|
Dh5a:pJP-3 | 22.5.13 | 5 mL | LB + 50 ug/mL Kan | 13 mL tube | 37 C, 225 RPM | ~ 13 h | 1 | ||
BW27784 | 1.6.13 | 5 mL | LB | 13 mL tube | 37 C, 225 RPM | ON | 7,7 | ||
BW27786 | 1.6.13 | 5 mL | LB | 13 mL tube | 37 C, 225 RPM | ON | 2,9 |
Educational
http://media.hhmi.org/biointeractive/vlabs/bacterial_id/index.html