User:Jarle Pahr/Microbiology: Difference between revisions

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{| class="wikitable" border="1" cellpadding="5" cellspacing="0"
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! Culture!! Date !! Volume !! Medium !! Container !! Incubation conditions !! Incubation time !! OD600 !! # of samples used for measuring OD600!!  Comment
! Strain/plasmid!! Date !! Volume !! Medium !! Container !! Incubation conditions !! Incubation time !! OD600 !! # of samples used for measuring OD600!!  Comment
|-
|-
| Dh5a:pJP-3 || 22.5.13  || 5 mL || LB + 50 ug/mL Kan || 13 mL tube ||37 C, 225 RPM ||~ 13 h || ||1 ||
| Dh5a:pJP-3 || 22.5.13  || 5 mL || LB + 50 ug/mL Kan || 13 mL tube ||37 C, 225 RPM ||~ 13 h || ||1 ||
|-
|BW27784||1.6.13||5 mL|| LB||13 mL tube||37 C, 225 RPM|| ON||7,7||
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||BW27786||1.6.13||5 mL|| LB||13 mL tube||37 C, 225 RPM|| ON||2,9||
|}
|}


=Educational=
=Educational=

Revision as of 06:38, 2 June 2013

Notes on microbiology:


http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0059310



When Is a Microbial Culture “Pure”? Persistent Cryptic Contaminant Escapes Detection Even with Deep Genome Sequencing: http://mbio.asm.org/content/4/2/e00591-12.abstract


http://cellculture.bitesizebio.com/articles/cell-culture-passage-number-explained/


Concepts/Glossary

  • Passage number


Strain collections

American Type Culture Collection (ATCC): http://www.lgcstandards-atcc.org/Products/Cells%20and%20Microorganisms/Primary%20Cells.aspx?geo_country=no

NCMA (Previously CCMP): https://ncma.bigelow.org/


Microbiology-themed blogs: http://www.microbe.net/microbiology-blogs/



Growth data

Growth data from cultures:


Strain/plasmid Date Volume Medium Container Incubation conditions Incubation time OD600 # of samples used for measuring OD600 Comment
Dh5a:pJP-3 22.5.13 5 mL LB + 50 ug/mL Kan 13 mL tube 37 C, 225 RPM ~ 13 h 1
BW27784 1.6.13 5 mL LB 13 mL tube 37 C, 225 RPM ON 7,7
BW27786 1.6.13 5 mL LB 13 mL tube 37 C, 225 RPM ON 2,9

Educational

http://media.hhmi.org/biointeractive/vlabs/bacterial_id/index.html

Handbooks and references

http://kunde.datakvalitet.no/SIHF/Mikrobiologi/