User:Jarle Pahr/Kits

Commercial kits for applications in molecular biology:

HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm (available at http://www.bioquote.com/product.php?id=291680)

PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1

Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg

Kit	Use	Advantages	Disadvantages	Time	Experience
Promega Wizard SV miniprep	DNA isolation	Quick	Low yield
Machery-Nagel NucleoBond Xtra Midi (midiprep)	DNA isolation	High yield (~20 ug)	Slow	2 d	Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet.
Qiaquick gel extraction	DNA extraction from gel	Fast	Impure product		Impurities give absorbption peak at 230 nm in eluted product.
Qiaquick PCR purification	Purification of PCR products	Fast
QIAEX II gel extraction	DNA extraction from gel	Higher purity than qiaquick	Slower than Quiaquick		Yield of about 200 ng from ~1.5 ug starting amount of plasmid.
QIAGEN PCR Cloningplus Kit	Subcloning (TA cloning)	?	?	?