User:Jarle Pahr/Kits

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Commercial kits for applications in molecular biology:
Commercial kits for applications in molecular biology:
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Promega Wizard SV Miniprep:
 
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Machery-Nagel NucleoBond Xtra Midi (midiprep):
 
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Quiaquick Gel Extraction kit
 
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Qiaquick PCR purification kit
 
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QIAex II Gel extraction kit.
 
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Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
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{| class="wikitable" border="1" cellpadding="5" cellspacing="0"
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|-
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! Kit!! Use !! Advantages !! Disadvantages !! Time  !! Experience
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|-
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| Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || ||
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|-
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| Machery-Nagel NucleoBond Xtra Midi (midiprep)||  DNA isolation ||High yield (~20 ug) || Slow || 2 d || 
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|  Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product ||  ||
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|  Qiaquick PCR purification|| Purification of PCR products || Fast||  || ||
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|  QIAEX II gel extraction|| DNA extraction from gel  || Higher purity than qiaquick || Slower than Quiaquick|| ||
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|}

Revision as of 08:14, 25 February 2013

Commercial kits for applications in molecular biology:


HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm (available at http://www.bioquote.com/product.php?id=291680)

PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1

Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg


Kit Use Advantages Disadvantages Time Experience
Promega Wizard SV miniprep DNA isolation Quick Low yield
Machery-Nagel NucleoBond Xtra Midi (midiprep) DNA isolation High yield (~20 ug) Slow 2 d
Qiaquick gel extraction DNA extraction from gel Fast Impure product
Qiaquick PCR purification Purification of PCR products Fast
QIAEX II gel extraction DNA extraction from gel Higher purity than qiaquick Slower than Quiaquick
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