User:Jarle Pahr/Kits: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jarle Pahr (talk | contribs) No edit summary |
Jarle Pahr (talk | contribs) No edit summary |
||
Line 17: | Line 17: | ||
| Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || || | | Promega Wizard SV miniprep || DNA isolation || Quick || Low yield || || | ||
|- | |- | ||
| Machery-Nagel NucleoBond Xtra Midi (midiprep)|| DNA isolation ||High yield (~20 ug) || Slow || 2 d || Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. | | Machery-Nagel NucleoBond Xtra Midi (midiprep)|| DNA isolation ||High yield (~20 ug), pure product. || Slow || 2 d || Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. | ||
|- | |- | ||
| Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product || ||Impurities give absorbption peak at 230 nm in eluted product. | | Qiaquick gel extraction|| DNA extraction from gel || Fast|| Impure product || ||Impurities give absorbption peak at 230 nm in eluted product. |
Revision as of 07:56, 11 March 2013
Commercial kits for applications in molecular biology:
HisExpress protein purification columns: http://www.claremontbio.com/HisExpress_Protein_Purification_Columns_s/72.htm
(available at http://www.bioquote.com/product.php?id=291680)
PrepEase his-purification: http://www.affymetrix.com/estore/browse/brand/usb/product.jsp?navMode=34000&productId=131366&navAction=jump&aId=productsNav#1_1
Affinity chromatography products at Jena Bioscience: http://www.jenabioscience.com/cms/en/1/browse/1450_affinity_chromatography.html?gclid=CJCPmK6HvrUCFQtY3godFWEAtg
Kit | Use | Advantages | Disadvantages | Time | Experience |
---|---|---|---|---|---|
Promega Wizard SV miniprep | DNA isolation | Quick | Low yield | ||
Machery-Nagel NucleoBond Xtra Midi (midiprep) | DNA isolation | High yield (~20 ug), pure product. | Slow | 2 d | Yields of 100-140 ng/uL in 200 uL, for a total of about 20 ug. Yield may depend on skill in handling washing and resuspension of DNA pellet. |
Qiaquick gel extraction | DNA extraction from gel | Fast | Impure product | Impurities give absorbption peak at 230 nm in eluted product. | |
Qiaquick PCR purification | Purification of PCR products | Fast | |||
QIAEX II gel extraction | DNA extraction from gel | Higher purity than qiaquick | Slower than Quiaquick | Yield of about 200 ng from ~1.5 ug starting amount of plasmid. | |
QIAGEN PCR Cloningplus Kit | Subcloning (TA cloning) | ? | ? | ? |